In vivo labeling of rabbit cholinergic basal forebrain neurons with fluorochromated antibodies

被引：0
|
作者：
Härtig, W
Varga, C
Kacza, J
Grosche, J
Seeger, J
Luiten, PGM
Brauer, K
Harkany, T
机构：
[1] Univ Leipzig, Paul Flechsig Inst Brain Res, D-04109 Leipzig, Germany
[2] Univ Leipzig, Dept Anat Histol & Embryol, D-04103 Leipzig, Germany
[3] Univ Groningen, Dept Mol Neurobiol, NL-9750 AA Haren, Netherlands
[4] Univ Szeged, Fac Med, Dept Med Chem, Szeged, Hungary
来源：
NEUROREPORT | 2002年 / 13卷 / 11期
关键词：
choline acetyltransferase; cholinergic system; low-affinity neurotrophin receptor; oxygen-enriched photoconversion;
D O I：
暂无
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Cholinergic basal forebrain neurons (CBFN) expressing the low-affinity neurotrophin receptor p75 (p75(NTR)) were previously selectively labeled in vivo with carbocyanine 3 (Cy3)-tagged anti-p75(NTR), but the applied 192IgG-conjugates recognized p75(NTR) only in rat The antibody ME 20.4 raised against human p75(NTR) had been shown to cross-react with the receptor in monkey, raccoon, sheep, cat, dog, pig and rabbit. Hence, for in vivo labeling of rabbit CBFN in the present study, ME 20.4 was fluorochromated with Cy3-N-hydroxysuccinimide ester and purified Cy3-ME 20.4 was injected intracerebroventricularly. Two days post-injection, clusters of Cy3-ME 20.4 were found in CBFN displaying choline acetyltransferase-immunoreactivity. Following photoconversion, electron microscopy revealed fluorochromated antibodies in secondary lysosomes. In conclusion, Cy3-ME 20.4 might become an appropriate marker for CBFN in live and fixed tissues of various mammalian species.
引用
收藏
页码：1395 / 1398
页数：4
相关论文
共 50 条
  • [41] ALTERED ACTIVATION OF BASAL FOREBRAIN CHOLINERGIC NEURONS IN EXPERIMENTAL SEPSIS
    Jimenez, D. M.
    Abraham, M. N.
    Pavlov, V. A.
    Deutschman, C. S.
    SHOCK, 2016, 45 (06): : 139 - 139
  • [42] On the role of the basal forebrain cholinergic neurons in regulation of recovery sleep
    Kalinchuk, A
    Stenberg, D
    Rosenberg, PA
    Porkka-Heiskanen, T
    SLEEP, 2005, 28 : A21 - A21
  • [43] Direct, complex effects of estrogens on basal forebrain cholinergic neurons
    Bora, SH
    Liu, ZP
    Kecojevic, A
    Merchenthaler, I
    Koliatsos, VE
    EXPERIMENTAL NEUROLOGY, 2005, 194 (02) : 506 - 522
  • [44] The role of basal forebrain cholinergic neurons in fear and extinction memory
    Knox, Dayan
    NEUROBIOLOGY OF LEARNING AND MEMORY, 2016, 133 : 39 - 52
  • [45] Impact of Basal Forebrain Cholinergic Inputs on Basolateral Amygdala Neurons
    Unal, Cagri T.
    Pare, Denis
    Zaborszky, Laszlo
    JOURNAL OF NEUROSCIENCE, 2015, 35 (02): : 853 - 863
  • [46] SURVIVAL OF ADULT BASAL FOREBRAIN CHOLINERGIC NEURONS AFTER LOSS OF TARGET NEURONS
    SOFRONIEW, MV
    GALLETLY, NP
    ISACSON, O
    SVENDSEN, CN
    SCIENCE, 1990, 247 (4940) : 338 - 342
  • [47] Reversal of spontaneous atrophy of cholinergic basal forebrain neurons in the aged primate by ex vivo gene therapy
    Smith, DE
    Roberts, J
    Gage, F
    Tuszynski, MH
    BIOLOGICAL PSYCHIATRY, 2000, 47 (08) : 7S - 8S
  • [48] CY3-conjugated antibodies directed against p75NTR for the in vivo labelling of rat basal forebrain cholinergic neurons
    Härtig, W
    Kacza, J
    Grosche, J
    Brauer, K
    Brückner, G
    Seeger, J
    Harkany, T
    JOURNAL OF NEUROCHEMISTRY, 2001, 78 : 61 - 61
  • [49] BASAL FOREBRAIN NEURONS PROVIDE MAJOR CHOLINERGIC INNERVATION OF PRIMATE NEOCORTEX
    STRUBLE, RG
    LEHMANN, J
    MITCHELL, SJ
    MCKINNEY, M
    PRICE, DL
    COYLE, JT
    DELONG, MR
    NEUROSCIENCE LETTERS, 1986, 66 (02) : 215 - 220
  • [50] Cooperative effects of sonic hedgehog and NGF on basal forebrain cholinergic neurons
    Reilly, JO
    Karavanova, ID
    Williams, KP
    Mahanthappa, NK
    Allendoerfer, KL
    MOLECULAR AND CELLULAR NEUROSCIENCE, 2002, 19 (01) : 88 - 96
12345