Carbon nanotube-based transducers for immunoassays

被引：34

作者：

Lynam, Carol ^{[1
,2
,3
]}

Gilmartin, Niamh ^{[1
,2
]}

Minett, Andrew I. ^{[3
]}

O'Kennedy, Richard ^{[1
,2
]}

Wallace, Gordon ^{[3
]}

机构：

[1] Dublin City Univ, Natl Ctr Sensor Res, Biomed Diagnost Inst, Dublin 9, Ireland

[2] Dublin City Univ, Natl Ctr Sensor Res, Sch Biotechnol, Dublin 9, Ireland

[3] Univ Wollongong, Intelligent Polymer Res Inst, ARC Ctr Excellence Electromat Sci, Wollongong, NSW 2522, Australia

来源：

CARBON | 2009年 / 47卷 / 10期

基金：

澳大利亚研究理事会; 爱尔兰科学基金会;

关键词：

DIRECT ELECTROCHEMISTRY; BIOSENSORS; ELECTRODE; BEHAVIOR; FUNCTIONALIZATION; IMMOBILIZATION;

D O I：

10.1016/j.carbon.2009.04.017

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The attachment of mouse immunoglobulin G (IgG) and anti-mouse IgG antibodies onto carbon nanotubes (CNTs), using either non-covalent or covalent means was investigated. The resultant CNTs were characterised using a variety of techniques including enzyme-linked and fluorescence-linked immunoassays, UV-visible-NIR and Raman spectroscopy, transmission electron microscopy and cyclic voltammetry. TEM images of the adsorbed antibody on the CNTs show that the covalent modification approach was successful, whereas the non-covalent approach resulted in no electrochemically detectable labelled antibody. Direct electrical communication between CNTs covalently linked to peroxidase-labelled antibodies was observed during cyclic voltammetry, which suggests applications in developing carbon-nanotube-based immunosensors. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：2337 / 2343

页数：7

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