Carbon nanotube-based transducers for immunoassays

被引：34
|
作者：
Lynam, Carol [1 ,2 ,3 ]
Gilmartin, Niamh [1 ,2 ]
Minett, Andrew I. [3 ]
O'Kennedy, Richard [1 ,2 ]
Wallace, Gordon [3 ]
机构：
[1] Dublin City Univ, Natl Ctr Sensor Res, Biomed Diagnost Inst, Dublin 9, Ireland
[2] Dublin City Univ, Natl Ctr Sensor Res, Sch Biotechnol, Dublin 9, Ireland
[3] Univ Wollongong, Intelligent Polymer Res Inst, ARC Ctr Excellence Electromat Sci, Wollongong, NSW 2522, Australia
来源：
CARBON | 2009年 / 47卷 / 10期
基金：
澳大利亚研究理事会; 爱尔兰科学基金会;
关键词：
DIRECT ELECTROCHEMISTRY; BIOSENSORS; ELECTRODE; BEHAVIOR; FUNCTIONALIZATION; IMMOBILIZATION;
D O I：
10.1016/j.carbon.2009.04.017
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
The attachment of mouse immunoglobulin G (IgG) and anti-mouse IgG antibodies onto carbon nanotubes (CNTs), using either non-covalent or covalent means was investigated. The resultant CNTs were characterised using a variety of techniques including enzyme-linked and fluorescence-linked immunoassays, UV-visible-NIR and Raman spectroscopy, transmission electron microscopy and cyclic voltammetry. TEM images of the adsorbed antibody on the CNTs show that the covalent modification approach was successful, whereas the non-covalent approach resulted in no electrochemically detectable labelled antibody. Direct electrical communication between CNTs covalently linked to peroxidase-labelled antibodies was observed during cyclic voltammetry, which suggests applications in developing carbon-nanotube-based immunosensors. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码：2337 / 2343
页数：7
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