Development of a rapid and reliable single-tube multiplex real-time PCR method for HLA-A*24:02 genotyping

被引：3
|
作者：
Wang, Yanxia [1 ,2 ]
Zhang, Tingting [1 ,2 ]
Zhang, Lirong [1 ,2 ]
Pei, Yanrui [1 ,2 ]
Zhao, Lili [1 ,2 ]
Li, Yanwei [1 ,2 ]
Liu, Lin [1 ]
Wang, Huijuan [1 ,2 ]
机构：
[1] Northwest Univ, Sch Life Sci, Xian 710069, Shaanxi, Peoples R China
[2] Natl Engn Res Ctr Miniaturized Detect Syst, Xian 710069, Shaanxi, Peoples R China
来源：
PHARMACOGENOMICS | 2019年 / 20卷 / 11期
基金：
中国国家自然科学基金;
关键词：
ARMS; aromatic antiepileptic drugs (AEDs); cutaneous adverse drug reactions (cADRs); HLA-A*24:02; qPCR; STEVENS-JOHNSON-SYNDROME; TOXIC EPIDERMAL NECROLYSIS; HLA-B LOCUS; CUTANEOUS ADVERSE-REACTIONS; CHAIN-REACTION ASSAY; HLA-B-ASTERISK-1502; ALLELE; JAPANESE PATIENTS; GENETIC-MARKERS; DRUG-REACTIONS; CARBAMAZEPINE;
D O I：
10.2217/pgs-2019-0052
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Aim:HLA-A*24:02 is significantly associated with cutaneous adverse drug reactions caused by aromatic antiepileptic drugs. Here, we aimed to establish a fast and reliable detection method for HLA-A*24:02 genotyping. Methods: A single-tube multiplex quantitative real-time polymerase chain reaction(qPCR) assay for HLA-A*24:02 genotyping was established by combining allele-specific primers with TaqMan probes. Results: A 100% concordance was observed between qPCR and SBT result in 106 Han subjects. The detection limit of the new method was 0.05ng DNA. The positive rate of HLA-A*24:02 in Tibetans (55.6%, n=81) was significantly higher than those in Han (34%, n=106), Uighur (27.5%, n=102), Bouyei (25.9%, n=116) and Miao populations (26.5%, n=113). Conclusion: The newly established qPCR assay was reliable for HLA-A*24:02 screening in clinical applications.
引用
收藏
页码：803 / 812
页数：10
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