A single-tube multiplex real-time PCR for HLA-B*38:02 genotype by detecting highly specific SNPs

被引：18
|
作者：
Wang, Fei [1 ]
Li, Wenqi [1 ]
Wang, Xuan [1 ]
Luo, Xiang [2 ]
Dai, Penggao [1 ,3 ]
机构：
[1] Northwest Univ Xian, Coll Life Sci, Natl Engn Res Ctr Miniaturized Detect Syst, Xian 710069, Shaanxi, Peoples R China
[2] Tongchuan Peoples Hosp Tongchuan, Dept Resp, Tongchuan, Shaanxi, Peoples R China
[3] Shaanxi Lifegen Co Ltd, Collaborat Innovat Port, Fengdong New City,Xixian New Area,Bldg 1, Xian 712000, Shaanxi, Peoples R China
来源：
PHARMACOGENOMICS | 2023年 / 24卷 / 01期
关键词：
agranulocytosis; ethnic group; HLA-B*38; 02; real-time PCR; thioamide antithyroid drugs; CHAIN-REACTION ASSAY; HLA-B; INDUCED AGRANULOCYTOSIS; SEQUENCE; ASSOCIATION; ANTIGEN;
D O I：
10.2217/pgs-2022-0132
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Purpose: HLA-B*38:02 is closely related to carbimazole/methimazole-induced agranulocytosis. This study aimed to develop and validate a rapid and economical method for HLA-B*38:02 genotyping. Methods: A single-tube multiplex real-time PCR detection system comprising amplification refractory mutation system primers and TaqMan probes was established for HLA-B*38:02 genotyping. Sequence-based typing was applied to validate the accuracy of the assay. Results: The accuracy of the assay was 100% for HLA-B*38:02 genotyping. The detection limit of the new method was 0.05 ng DNA. The positive rate of HLA-B*38:02 in the Han (8%, n = 100), Bouyei (17.8%, n = 90) and Tibetan (12.7%, n = 110) populations was significantly higher than that in the Uighur population (1%, n = 100) (p < 0.05). Conclusion: The proposed method is rapid and reliable for HLA-B*38:02 screening in a clinical setting.
引用
收藏
页码：5 / 14
页数：10
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