RNAi Codex: a portal/database for short-hairpin RNA (shRNA) gene-silencing constructs

被引：38
|
作者：
Olson, A. [1 ]
Sheth, N. [1 ]
Lee, J. S. [1 ]
Hannon, G. [1 ]
Sachidanandam, R. [1 ]
机构：
[1] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA
来源：
NUCLEIC ACIDS RESEARCH | 2006年 / 34卷
关键词：
D O I：
10.1093/nar/gkj051
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Use of RNA interference (RNAi) in forward genetic screens is proliferating. Currently, short-interfering RNAs (siRNAs) and short-hairpin RNAs (shRNAs) are being used to silence genes to tease out functional information. It is becoming easier to harness RNAi to silence specific genes, owing to the development of libraries of readymade shRNA and siRNA gene-silencing constructs by using a variety of sources. RNAi Codex, which consists of a database of shRNA related information and an associated website, has been developed as a portal for publicly available shRNA resources and is accessible at http://codex.cshl.org. RNAi Codex currently holds data from the Hannon-Elledge shRNA library and allows the use of biologist-friendly gene names to access information on shRNA constructs that can silence the gene of interest. It is designed to hold user-contributed annotations and publications for each construct, as and when such data become available. We will describe features of RNAi Codex and explain the use of the tool.
引用
收藏
页码：D153 / D157
页数：5
相关论文
共 50 条
  • [31] Antisense and short hairpin RNA (shRNA) constructs targeting PIN (protein inhibitor of NOS) ameliorate aging-related erectile dysfunction in the rat
    Magee, Thomas R.
    Kovanecz, Istvan
    Davila, Hugo H.
    Ferrini, Monica G.
    Cantini, Liliana
    Vernet, Dolores
    Zuniga, Freddi I.
    Rajfer, Jacob
    Gonzalez-Cadavid, Nestor F.
    JOURNAL OF SEXUAL MEDICINE, 2007, 4 (03): : 633 - 643
  • [32] Comparison of approaches for efficient gene silencing induced by microRNA-based short hairpin RNA and indicator gene expression
    Z. X. Shan
    Q. X. Lin
    C. Y. Deng
    Z. L. Zhou
    H. H. Tan
    Y. H. Fu
    X. H. Li
    J. N. Zhu
    L. P. Mai
    S. J. Kuang
    S. G. Lin
    X. Y. Yu
    Molecular Biology Reports, 2010, 37 : 1831 - 1839
  • [33] Comparison of approaches for efficient gene silencing induced by microRNA-based short hairpin RNA and indicator gene expression
    Shan, Z. X.
    Lin, Q. X.
    Deng, C. Y.
    Zhou, Z. L.
    Tan, H. H.
    Fu, Y. H.
    Li, X. H.
    Zhu, J. N.
    Mai, L. P.
    Kuang, S. J.
    Lin, S. G.
    Yu, X. Y.
    MOLECULAR BIOLOGY REPORTS, 2010, 37 (04) : 1831 - 1839
  • [34] Targeted suppression of Has2 mRNA in mouse cumulus cell-oocyte complexes (COCs) by adenovirus-mediated short-hairpin RNA (shRNA) expression
    Sugiura, Koji
    Eppig, John
    BIOLOGY OF REPRODUCTION, 2008, : 127 - 127
  • [35] Cell cycle arrest drastically extends the duration of gene silencing after transient expression of short hairpin RNA
    Maliyekkel, Anil
    Davis, Brian M.
    Roninson, Igor B.
    CELL CYCLE, 2006, 5 (20) : 2390 - 2395
  • [36] Silencing HBV gene expression with Pol II and Pol III promoter-derived short hairpin RNA
    Carmona, S
    Ely, A
    Moolla, N
    Weinberg, M
    Arbuthnot, P
    MOLECULAR THERAPY, 2004, 9 : S208 - S208
  • [37] Short hairpin type of dsRNAs that are controlled by tRNAVal promoter significantly induce RNAi-mediated gene silencing in the cytoplasm of human cells
    Kawasaki, H
    Taira, K
    NUCLEIC ACIDS RESEARCH, 2003, 31 (02) : 700 - 707
  • [38] Short-Hairpin RNA-Mediated MTA2 Silencing Inhibits Human Breast Cancer Cell Line MDA-MB231 Proliferation and Metastasis
    Lu, Jun
    Jin, Mu-Lan
    ASIAN PACIFIC JOURNAL OF CANCER PREVENTION, 2014, 15 (14) : 5577 - 5582
  • [39] Short Hairpin RNA Gene Silencing of Prolyl Hydroxylase-2 with a Minicircle Vector Improves Neovascularization of Hindlimb Ischemia
    Lijkwan, Maarten A.
    Hellingman, Alwine A.
    Bos, Ernst J.
    van der Bogt, Koen E. A.
    Huang, Mei
    Kooreman, Nigel G.
    de Vries, Margreet R.
    Peters, Hendrika A. B.
    Robbins, Robert C.
    Hamming, Jaap F.
    Quax, Paul H. A.
    Wu, Joseph C.
    HUMAN GENE THERAPY, 2014, 25 (01) : 41 - 49
  • [40] Short hairpin RNA-mediated matrix gene silencing of human respiratory syncytial virus as a potent antiviral strategy
    Fard, Saeid Amiri Zadeh
    Abuei, Haniyeh
    Farhadi, Ali
    Dehbidi, Gholamreza Rafiei
    Zare, Farahnaz
    Abbasfard, Zahra
    Behbahani, Abbas Behzad
    FUTURE VIROLOGY, 2023, 18 (05) : 309 - 319
12345