RNAi Codex: a portal/database for short-hairpin RNA (shRNA) gene-silencing constructs

被引：38
|
作者：
Olson, A. [1 ]
Sheth, N. [1 ]
Lee, J. S. [1 ]
Hannon, G. [1 ]
Sachidanandam, R. [1 ]
机构：
[1] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA
来源：
NUCLEIC ACIDS RESEARCH | 2006年 / 34卷
关键词：
D O I：
10.1093/nar/gkj051
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Use of RNA interference (RNAi) in forward genetic screens is proliferating. Currently, short-interfering RNAs (siRNAs) and short-hairpin RNAs (shRNAs) are being used to silence genes to tease out functional information. It is becoming easier to harness RNAi to silence specific genes, owing to the development of libraries of readymade shRNA and siRNA gene-silencing constructs by using a variety of sources. RNAi Codex, which consists of a database of shRNA related information and an associated website, has been developed as a portal for publicly available shRNA resources and is accessible at http://codex.cshl.org. RNAi Codex currently holds data from the Hannon-Elledge shRNA library and allows the use of biologist-friendly gene names to access information on shRNA constructs that can silence the gene of interest. It is designed to hold user-contributed annotations and publications for each construct, as and when such data become available. We will describe features of RNAi Codex and explain the use of the tool.
引用
收藏
页码：D153 / D157
页数：5
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