Cloning and analyzing of the cDNA sequence of N-terminal region and C-terminal region of zinc finger protein (ZFP580) gene

被引：0
|
作者：
Zhu, Yebin [1 ,2 ]
Zhang, Wencheng [1 ]
机构：
[1] Med Coll Armed Police Force, Physiol & Pathol Lab, Tianjin 300162, Peoples R China
[2] Hebei Med Univ, Shijiazhuang 050017, Peoples R China
来源：
LIFE SCIENCE JOURNAL-ACTA ZHENGZHOU UNIVERSITY OVERSEAS EDITION | 2008年 / 5卷 / 01期
关键词：
ZFP580; gene cloning; atherosclerosis; C2H2 zinc finger;
D O I：
暂无
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Objective. To clone and analyze the cDNA sequence of N-terminal region and C-terminal region of zinc finger protein (ZFP580) gene from rat cerebellum. Methods. Total RNA was extracted from rat cerebellum. The two cDNA fragment encoding the N-terminal region and C-terminal region of ZFP580 was amplified by RT-PCR with specific primers, cloned into pMD 18-T-Easy vector and then sequenced, respectively. Results. The two fragments were obtained by RT-PCR successfully. DNA sequencing result showed that the two fragments were exactly consistent with the sequence reported in gene bank. Conclusion. The results showed that the N-terminal region and C-terminal region of ZFP580 was successfully cloned, which lays a reliable foundation for further research of genetically engineered drugs and the gene therapies for atherosclerosis. [Life Science Journal. 2008; 5(1): 11 - 16] (ISSN: 1097 - 8135).
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页码：11 / 16
页数：6
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