Cloning and analyzing of the cDNA sequence of N-terminal region and C-terminal region of zinc finger protein (ZFP580) gene

Objective. To clone and analyze the cDNA sequence of N-terminal region and C-terminal region of zinc finger protein (ZFP580) gene from rat cerebellum. Methods. Total RNA was extracted from rat cerebellum. The two cDNA fragment encoding the N-terminal region and C-terminal region of ZFP580 was amplified by RT-PCR with specific primers, cloned into pMD 18-T-Easy vector and then sequenced, respectively. Results. The two fragments were obtained by RT-PCR successfully. DNA sequencing result showed that the two fragments were exactly consistent with the sequence reported in gene bank. Conclusion. The results showed that the N-terminal region and C-terminal region of ZFP580 was successfully cloned, which lays a reliable foundation for further research of genetically engineered drugs and the gene therapies for atherosclerosis. [Life Science Journal. 2008; 5(1): 11 - 16] (ISSN: 1097 - 8135).