Identification of fungal pathogens from clinical specimens using multi-locus PCR coupled with electrospray ionization mass spectrometry

被引：4
|
作者：
Wu, Chi-Jung [1 ,2 ,3 ]
Chen, Ya-Ping [2 ,3 ]
Wang, Hsuan-Chen [1 ]
Su, Ih-Jen [1 ]
Ko, Wen-Chien [2 ,3 ]
Chen, Jiann-Shiuh [3 ,4 ]
Cheng, Chao-Neng [3 ,4 ]
Lee, Nan-Yao [2 ,3 ]
Sun, H. Sunny [5 ]
Chi, Chia-Yu [1 ,3 ,4 ]
Chen, Tsai-Yun [2 ,3 ]
机构：
[1] Natl Inst Infect Dis & Vaccinol, Natl Hlth Res Inst, Tainan, Taiwan
[2] Natl Cheng Kung Univ Hosp, Dept Internal Med, Tainan 70428, Taiwan
[3] Natl Cheng Kung Univ, Coll Med, Tainan 70101, Taiwan
[4] Natl Cheng Kung Univ Hosp, Dept Pediat, Tainan 70428, Taiwan
[5] Natl Cheng Kung Univ, Coll Med, Inst Mol Med, Tainan 70101, Taiwan
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2014年 / 78卷 / 02期
关键词：
Electrospray ionization mass spectrometry; Fungal infection; PCR; Paraffin; DNA;
D O I：
10.1016/j.diagmicrobio.2013.08.007
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Polymerase chain reaction coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was successfully used to identify a variety of fungi, including mixed fungal species, from 10 of 12 clinical specimens (10 culture-negative) of biopsy tissues or body fluids from patients with fungal infections. The application of PCR/ESI-MS for identifying fungal pathogens is discussed. (C) 2014 Elsevier Inc. All rights reserved.
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页码：141 / 143
页数：3
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