Identification of fungal pathogens from clinical specimens using multi-locus PCR coupled with electrospray ionization mass spectrometry

被引：4

作者：

Wu, Chi-Jung ^{[1
,2
,3
]}

Chen, Ya-Ping ^{[2
,3
]}

Wang, Hsuan-Chen ^{[1
]}

Su, Ih-Jen ^{[1
]}

Ko, Wen-Chien ^{[2
,3
]}

Chen, Jiann-Shiuh ^{[3
,4
]}

Cheng, Chao-Neng ^{[3
,4
]}

Lee, Nan-Yao ^{[2
,3
]}

Sun, H. Sunny ^{[5
]}

Chi, Chia-Yu ^{[1
,3
,4
]}

Chen, Tsai-Yun ^{[2
,3
]}

机构：

[1] Natl Inst Infect Dis & Vaccinol, Natl Hlth Res Inst, Tainan, Taiwan

[2] Natl Cheng Kung Univ Hosp, Dept Internal Med, Tainan 70428, Taiwan

[3] Natl Cheng Kung Univ, Coll Med, Tainan 70101, Taiwan

[4] Natl Cheng Kung Univ Hosp, Dept Pediat, Tainan 70428, Taiwan

[5] Natl Cheng Kung Univ, Coll Med, Inst Mol Med, Tainan 70101, Taiwan

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2014年 / 78卷 / 02期

关键词：

Electrospray ionization mass spectrometry; Fungal infection; PCR; Paraffin; DNA;

D O I：

10.1016/j.diagmicrobio.2013.08.007

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Polymerase chain reaction coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was successfully used to identify a variety of fungi, including mixed fungal species, from 10 of 12 clinical specimens (10 culture-negative) of biopsy tissues or body fluids from patients with fungal infections. The application of PCR/ESI-MS for identifying fungal pathogens is discussed. (C) 2014 Elsevier Inc. All rights reserved.

引用

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页码：141 / 143

页数：3

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