Effects of garlic components diallyl sulfide and diallyl disulfide on arylamine N-acetyltransferase activity and 2-aminofluorene-DNA adducts in human promyelocytic leukemia cells

被引：21
|
作者：
Lin, JG [1 ]
Chen, GW [1 ]
Su, CC [1 ]
Hung, CF [1 ]
Yang, CC [1 ]
Lee, JH [1 ]
Chung, JG [1 ]
机构：
[1] China Med Coll, Dept Med, Inst Chinese Med Sci, Taichung 400, Taiwan
来源：
AMERICAN JOURNAL OF CHINESE MEDICINE | 2002年 / 30卷 / 2-3期
关键词：
diallyl sulfide; diallyl disulfide; 2-aminofluorene; HL-60; NAT;
D O I：
10.1142/S0192415X02000338
中图分类号：
R [医药、卫生];
学科分类号：
10 ;
摘要：
Two components of garlic, diallyl sulfide (DAS) and diallyl disulfide (DADS), inhibited arylamine N-acetyltransferase (NAT) activity and 2-aminofluorene-DNA adduct in human promyelocytic leukemia cells (HL-60). The NAT activity was measured by high performance liquid chromatography assaying for amounts of N-acetyl-2-aminofluorene (2-AAF) and remaining 2-aminofluorene (2-AF). Cellular cytosols and intact cell suspensions were assayed, The inhibition of NAT activity and 2-AF-DNA adduct formation in human leukemia cells by DAS and DADS were dose-dependent and were directly proportional. The data also indicated that DAS and DADS decrease the apparent values of K-m and V-max from human leukemia cells in both assays. This is the first report of garlic components affecting human leukemia cell NAT activity and 2-AF-DNA adduct formation.
引用
收藏
页码：315 / 325
页数：11
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