Study on rolling circle amplification of Ebola virus and fluorescence detection based on graphene oxide

被引：46
|
作者：
Wen, Jia [1 ]
Li, Weisi [1 ]
Li, Jiaqi [1 ]
Tao, Binbin [1 ]
Xu, Yongqian [1 ]
Li, Hongjuan [1 ]
Lu, Aiping [2 ]
Sun, Shiguo [1 ]
机构：
[1] Northwest A&F Univ, Coll Sci, Yangling 712100, Shaanxi, Peoples R China
[2] Hong Kong Baptist Univ, Sch Chinese Med, Kowloon Tong 999077, Hong Kong, Peoples R China
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2016年 / 227卷
基金：
中国国家自然科学基金;
关键词：
Rolling circle amplification; Ebola virus; Graphene oxide; Fluorescence; ULTRASENSITIVE ELECTROCHEMICAL DETECTION; CASCADE SIGNAL AMPLIFICATION; COLOR QUANTUM DOTS; NUCLEIC-ACIDS; DNA DETECTION; LABEL-FREE; OLIGONUCLEOTIDE MICROARRAY; NUCLEOTIDE POLYMORPHISMS; BIOSENSING PLATFORM; STRATEGY;
D O I：
10.1016/j.snb.2016.01.036
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A graphene oxide (GO) assisted rolling circle amplification (RCA) platform for Ebola virus (EBOV) detection is developed with simplicity and high sensitivity. In the absence of EBOV gene, no RCA products generated and the fluorescein amidate (FAM) labeled detection probe was adsorbed on the surface of GO, resulting in fluorescence quenching of the FAM. Addition of the EBOV gene allowed RCA to be taken place and the formation of double-stranded DNA (dsDNA) between RCA products and FAM labeled detection probe, leading to desorption of the FAM labeled detection probe from GO surface accompanied fluorescence recovery. EBOV gene can be determined both in aqueous solution and 1% serum solution. The limit of detection was 1.4 pM. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码：655 / 659
页数：5
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