Rolling Circle Amplification-Based Biosensors

被引：8

作者：

Ou, Lijuan ^{[1
]}

Sun, Aiming ^{[1
]}

Liu, Kaijian ^{[1
]}

机构：

[1] Hunan Inst Technol, Coll Mat & Chem Engn, Hengyang 421002, Hunan, Peoples R China

来源：

ANALYTICAL LETTERS | 2015年 / 48卷 / 08期

关键词：

Biodiagnostics; Rolling circle amplification; Isothermal; SINGLE-NUCLEOTIDE POLYMORPHISMS; CASCADE SIGNAL AMPLIFICATION; HIGHLY SENSITIVE DETECTION; SURFACE-PLASMON RESONANCE; ULTRASENSITIVE ELECTROCHEMICAL DETECTION; APTAMERIC RECOGNITION SYSTEM; MOLECULE-LINKED DNA; PROTEIN-DETECTION; IN-SITU; POINT MUTATIONS;

D O I：

10.1080/00032719.2014.979354

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Rolling circle amplification is an isothermal method for amplifying DNA. The technique rapidly synthesizes a long (more than 1000 bases), linear, tandemly-repetitive single-stranded DNA chain by a short (less than 100 bases long) circular template. The reaction may proceed in either linear or exponential amplification modes and may provide copies of the circle up to 10(4)-fold. The robustness, speed, and sensitivity of the rolling circle amplification strategies have gained widespread application in DNA and protein assays, single nucleotide polymorphism, in situ signal amplification, drug discovery, and nanotechnology.

引用

页码：1199 / 1216

页数：18

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