Recent advancements in surface-enhanced laser desorption/ionization-time of flight-mass spectrometry

被引：78
|
作者：
Merchant, M [1 ]
Weinberger, SR [1 ]
机构：
[1] Ciphergen Biosyst Inc, Palo Alto, CA 94306 USA
来源：
ELECTROPHORESIS | 2000年 / 21卷 / 06期
关键词：
surface-enhanced laser desorption; ionization; review;
D O I：
10.1002/(SICI)1522-2683(20000401)21:6<1164::AID-ELPS1164>3.3.CO;2-S
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The overall history and recent advances in surface enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS) technology is reviewed herein. Fundamentals of SELDI-TOF analysis are presented while drawing comparisons with other laser-based mass spectrometry techniques. The application of SELDI-TOF-MS to functional genomics and biomarker discovery is discussed and exemplified by elucidating a biomarker candidate for prostatic carcinoma. Finally, a short discussion regarding future SELDI requirements and developments is supplied.
引用
收藏
页码：1164 / 1177
页数：14
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