Different Types of Aqueous Two-Phase Systems for Biomolecule and Bioparticle Extraction and Purification

被引：69

作者：

Dutra Molino, Joao Vitor ^{[1
]}

Viana Marques, Daniela de Araujo ^{[1
]}

Pessoa Junior, Adalberto ^{[1
]}

Mazzola, Priscila Gava ^{[2
]}

Viccari Gatti, Maria Silvia ^{[3
]}

机构：

[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, BR-05508000 Sao Paulo, Brazil

[2] Univ Estadual Campinas, Dept Clin Patol, Fac Med Sci, BR-13083887 Campinas, SP, Brazil

[3] Univ Estadual Campinas, Inst Biol, Genet Evolut & Bioagents Dept, BR-13083862 Campinas, SP, Brazil

来源：

BIOTECHNOLOGY PROGRESS | 2013年 / 29卷 / 06期

基金：

巴西圣保罗研究基金会;

关键词：

liquid-liquid extraction; aqueous two-phase system; bioparticles; biomolecules; LIQUID-LIQUID-EXTRACTION; NONIONIC MICELLAR SYSTEMS; VIRUS-LIKE-PARTICLES; COLI CELL LYSATE; PLASMID DNA; ESCHERICHIA-COLI; HUMAN-ANTIBODIES; ENVELOPE GLYCOPROTEIN; SELECTIVE PARTITION; SALVIA-MILTIORRHIZA;

D O I：

10.1002/btpr.1792

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Upstream improvements have led to significant advances in the productivity of biomolecules and bioparticles. Today, downstream processes are the bottleneck in the production of some biopharmaceuticals, a change from previous years. Current purification platforms will reach their physical limits at some point, indicating the need for new approaches. This article reviews an alternative method to extract and purify biomolecules/bioparticles named aqueous two-phase system (ATPS). Biocompatibility and readiness to scale up are some of the ATPS characteristics. We also discuss some of ATPS applications in the biotechnology field. (c) 2013 American Institute of Chemical Engineers Biotechnol. Prog., 29:1343-1353, 2013

引用

页码：1343 / 1353

页数：11

共 50 条

[41] Advances on aqueous two-phase extraction
Hu, Song-Qing
Li, Lin
Guo, Si-Yuan
Chen, Ling
Cai, Miao-Yan
Xiandai Huagong/Modern Chemical Industry, 2004, 24 (06): : 22 - 25
[42] Experiment and simulation of an aqueous two-phase extraction process for the purification of a monoclonal antibody
Muendges, Jan
Zierow, Jessica
Zeiner, Tim
CHEMICAL ENGINEERING AND PROCESSING-PROCESS INTENSIFICATION, 2015, 95 : 31 - 42
[43] Extraction and Purification of Lipoxygenase from Soybean Using Aqueous Two-Phase System
Lakshmi, M. C.
Madhusudhan, M. C.
Raghavarao, K. S. M. S.
FOOD AND BIOPROCESS TECHNOLOGY, 2012, 5 (01) : 193 - 199
[44] Affinity-enhanced purification of human antibodies by aqueous two-phase extraction
Azevedo, A. M.
Rosa, P. A. J.
Ferreira, I. F.
Pisco, A. M. M. O.
de Vries, J.
Korporaal, R.
Visser, T. J.
Aires-Barros, M. R.
SEPARATION AND PURIFICATION TECHNOLOGY, 2009, 65 (01) : 31 - 39
[45] Purification of proteins with aqueous two-phase solvent systems by countercurrent chromatography
Shibusawa, Y
Ito, Y
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, 1998, 28 (02): : 99 - 136
[46] Purification of human immunoglobulin G by thermoseparating aqueous two-phase systems
Ferreira, I. Filipa
Azevedo, Ana M.
Rosa, Paula A. J.
Aires-Barros, M. Raquel
JOURNAL OF CHROMATOGRAPHY A, 2008, 1195 (1-2) : 94 - 100
[47] Reactive aqueous two-phase systems for the production and purification of PEGylated proteins
Campos-Garcia, Victor R.
Benavides, Jorge
Gonzalez-Valdez, Jose
ELECTRONIC JOURNAL OF BIOTECHNOLOGY, 2021, 54 : 60 - 68
[48] Aqueous two-phase systems: Fundamentals and applications for partitioning/purification of proteins
Mendes da Silva, Luis Henrique
Loh, Watson
QUIMICA NOVA, 2006, 29 (06): : 1345 - 1351
[49] Review of Microbial Lipase Purification Using Aqueous Two-phase Systems
Show, Pau-Loke
Ling, Tau-Chuan
Lan, John C. -W.
Tey, Beng-Ti
Ramanan, Ramakrishnan N.
Yong, Siek-Ting
Ooi, Chien-Wei
CURRENT ORGANIC CHEMISTRY, 2015, 19 (01) : 19 - 29
[50] Two-phase aqueous micellar systems - An alternative method for protein purification
Rangel-Yagui, CO
Pessoa, A
Blankschtein, D
BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING, 2004, 21 (04) : 531 - 544

← 1 2 3 4 5 →