Different Types of Aqueous Two-Phase Systems for Biomolecule and Bioparticle Extraction and Purification

被引：69

作者：

Dutra Molino, Joao Vitor ^{[1
]}

Viana Marques, Daniela de Araujo ^{[1
]}

Pessoa Junior, Adalberto ^{[1
]}

Mazzola, Priscila Gava ^{[2
]}

Viccari Gatti, Maria Silvia ^{[3
]}

机构：

[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, BR-05508000 Sao Paulo, Brazil

[2] Univ Estadual Campinas, Dept Clin Patol, Fac Med Sci, BR-13083887 Campinas, SP, Brazil

[3] Univ Estadual Campinas, Inst Biol, Genet Evolut & Bioagents Dept, BR-13083862 Campinas, SP, Brazil

来源：

BIOTECHNOLOGY PROGRESS | 2013年 / 29卷 / 06期

基金：

巴西圣保罗研究基金会;

关键词：

liquid-liquid extraction; aqueous two-phase system; bioparticles; biomolecules; LIQUID-LIQUID-EXTRACTION; NONIONIC MICELLAR SYSTEMS; VIRUS-LIKE-PARTICLES; COLI CELL LYSATE; PLASMID DNA; ESCHERICHIA-COLI; HUMAN-ANTIBODIES; ENVELOPE GLYCOPROTEIN; SELECTIVE PARTITION; SALVIA-MILTIORRHIZA;

D O I：

10.1002/btpr.1792

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Upstream improvements have led to significant advances in the productivity of biomolecules and bioparticles. Today, downstream processes are the bottleneck in the production of some biopharmaceuticals, a change from previous years. Current purification platforms will reach their physical limits at some point, indicating the need for new approaches. This article reviews an alternative method to extract and purify biomolecules/bioparticles named aqueous two-phase system (ATPS). Biocompatibility and readiness to scale up are some of the ATPS characteristics. We also discuss some of ATPS applications in the biotechnology field. (c) 2013 American Institute of Chemical Engineers Biotechnol. Prog., 29:1343-1353, 2013

引用

页码：1343 / 1353

页数：11

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