Crystallization and preliminary X-ray crystallographic analysis of human nucleoside diphosphate kinase A

Human nucleoside diphosphate kinase A catalyzes phosphoryl transfer and acts as a suppressor of metastasis. It has been crystallized using 2-methyl-2,4-pentanediol as a precipitant at 288 K. The crystal is monoclinic, belonging to the space group P2(1), with unit-cell parameters a = 74.21, b = 78.11, c = 82.29 Angstrom, beta = 101.33 degrees. The asymmetric unit contains a homohexamer, with a corresponding crystal volume per protein mass (V-m) of 2.27 Angstrom(3) Da(-1) and a solvent content of 46%. Native X-ray data to 2.15 Angstrom resolution have been collected using synchrotron X-rays.