Simple method for determining nucleobase pKa values by indirect labeling and demonstration of a pKa of neutrality in dsDNA

被引：25

作者：

Moody, EM

Brown, TS

Bevilacqua, PC ^{[1
]}

机构：

[1] Penn State Univ, Dept Chem, University Pk, PA 16802 USA

[2] Penn State Univ, Huck Inst Life Sci, University Pk, PA 16802 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 33期

关键词：

D O I：

10.1021/ja047362h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Phosphorothioates were substituted into double-stranded DNA to study protonated Class I A+·C basepairs by 31P NMR. The method was effective in reporting the A+·C pKa. pKa values near 7.0 were found with optimal nearest-neighbor partners. Such pKa values could expand the catalytic repertoire of nucleic acids. Copyright © 2004 American Chemical Society.

引用

页码：10200 / 10201

页数：2

共 50 条

[31] Measurement of nucleobase pKa values in model mononucleotides shows RNA-RNA duplexes to be more stable than DNA-DNA duplexes
Acharya, P
Cheruku, P
Chatterjee, S
Acharya, S
Chattopadhyaya, J
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (09) : 2862 - 2869
[32] Measurement of Nucleobase pKa Values in Model Mononucleotides Shows RNA-RNA Duplexes to Be More Stable than DNA-DNA Duplexes
Chattopadhyaya, J. (jyoti@boc.uu.se), 1600, American Chemical Society (126):
[33] A SIMPLE METHOD FOR DETERMINING LABELING EFFICIENCY OF FLUORESCEIN ISOTHIOCYANATE PRODUCTS
MCKINNEY, RM
SPILLANE, JT
PEARCE, GW
ANALYTICAL BIOCHEMISTRY, 1966, 14 (03) : 421 - &
[34] Empirically Fitted Parameters for Calculating pKa Values with Small Deviations from Experiments Using a Simple Computational Strategy
Galano, Annia
Perez-Gonzalez, Adriana
Castaneda-Arriaga, Romina
Munoz-Rugeles, Leonardo
Mendoza-Sarmiento, Gabriela
Romero-Silva, Antonio
Ibarra-Escutia, Agustin
Mariana Rebollar-Zepeda, Aida
Rafael Leon-Carmona, Jorge
Hernandez-Olivares, Manuel Alejandro
Raul Alvarez-Idaboy, Juan
JOURNAL OF CHEMICAL INFORMATION AND MODELING, 2016, 56 (09) : 1714 - 1724
[35] A reliable and efficient first principles-based method for predicting pKa values. III. Adding explicit water molecules: Can the theoretical slope be reproduced and pKa values predicted more accurately?
Zhang, Shuming
JOURNAL OF COMPUTATIONAL CHEMISTRY, 2012, 33 (05) : 517 - 526
[36] The AIBLHiCoS Method: Predicting Aqueous pKa Values from Gas-Phase Equilibrium Bond Lengths
Anstoeter, Cate
Caine, Beth A.
Popelier, Paul L. A.
JOURNAL OF CHEMICAL INFORMATION AND MODELING, 2016, 56 (03) : 471 - 483
[37] Comprehensive Determination of Protein Tyrosine pKa Values for Photoactive Yellow Protein Using Indirect 13C NMR Spectroscopy
Oktaviani, Nur Alia
Pool, Trijntje J.
Kamikubo, Hironari
Slager, Jelle
Scheek, Ruud M.
Kataoka, Mikio
Mulder, Frans A. A.
BIOPHYSICAL JOURNAL, 2012, 102 (03) : 579 - 586
[38] A modified RP-HPLC method for the determination of the pKa values of synthesized β-hydroxy-β-arylalkanoic acids
Savic, Jelena S.
Dilber, Sanda P.
Vujic, Zorica B.
Vladimirov, Sote M.
Brboric, Jasmina S.
JOURNAL OF THE SERBIAN CHEMICAL SOCIETY, 2018, 83 (7-8) : 875 - 883
[39] SIMPLE METHOD FOR DETERMINING LABELING EFFICIENCY OF TECHNETIUM-SULFUR COLLOID
LEWIS, CL
BRAMLET, R
JOURNAL OF NUCLEAR MEDICINE, 1972, 13 (04) : 290 - &
[40] A SIMPLE RELATIONSHIP BETWEEN THE HAMMETT ACIDITY FUNCTION, H0, AND THE THERMODYNAMIC PKA VALUES OF UNSUBSTITUTED AROMATIC CARBOXAMIDES
LOVELL, MW
SCHULMAN, SG
ANALYTICA CHIMICA ACTA, 1981, 127 (JUN) : 203 - 207

← 1 2 3 4 5 →