Simple method for determining nucleobase pKa values by indirect labeling and demonstration of a pKa of neutrality in dsDNA

被引：25
|
作者：
Moody, EM
Brown, TS
Bevilacqua, PC [1 ]
机构：
[1] Penn State Univ, Dept Chem, University Pk, PA 16802 USA
[2] Penn State Univ, Huck Inst Life Sci, University Pk, PA 16802 USA
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 33期
关键词：
D O I：
10.1021/ja047362h
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Phosphorothioates were substituted into double-stranded DNA to study protonated Class I A+·C basepairs by 31P NMR. The method was effective in reporting the A+·C pKa. pKa values near 7.0 were found with optimal nearest-neighbor partners. Such pKa values could expand the catalytic repertoire of nucleic acids. Copyright © 2004 American Chemical Society.
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页码：10200 / 10201
页数：2
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