Functional study of Lactococcus lactis RNase III in Escherichia coli

被引:5
|
作者
Drider, D
Bolotine, A
Renault, P
Prévost, H
机构
[1] ENITIAA, Lab Microbiol Alimentaire & Ind, F-44322 Nantes 3, France
[2] INRA, Lab Genet Microbienne, F-75352 Jouy En Josas, France
关键词
Lactococcus lactis; RNase III; transcriptional analysis; heterologous complementation; E; coli;
D O I
10.1016/S0147-619X(02)00010-0
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Endoribonuclease III (RNase III) is known to participate in a number of RNA maturation and decay pathways. Previous research carried out on citQRP mRNA processing had provided strong evidence that RNase III exists in Lactococcus lactis and acts as a major endoribonuclease in the control of citQRP mRNA stability. The DNA coding (rnc) for the putative lactococcal RNase III was cloned by PCR technology. In addition, we provide the transcriptional analysis of me gene and compared the lactococcal RNase III sequence to those of Gram-positive and Gram-negative organisms. This computer-assisted comparison showed an identity ranking from 37% to 54'%, and the highest score was obtained with Streptococcus pneumoniae RNase III. Moreover, heterologous complementation indicated that lactococcal RNase III is able to complement the loss of Escherichia coli RNase III for both 30S RNA processing and lambda N posttranscriptional regulator. (C) 2002 Elsevier Science (USA). All rights reserved.
引用
收藏
页码:246 / 250
页数:5
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