In vivo imaging of Escherichia coli and Lactococcus lactis in murine intestines using a reporter luciferase gene

被引：6
|
作者：
Lee, Mi-Sook [2 ]
Moon, Gi-Seong [1 ]
机构：
[1] Chungju Natl Univ, Dept Biotechnol, Jeungpyeong 368701, Chungbuk, South Korea
[2] Korea Basic Sci Inst, Chuncheon Ctr, Div Bioimaging, Chunchon 200701, Gangwon, South Korea
来源：
FOOD SCIENCE AND BIOTECHNOLOGY | 2012年 / 21卷 / 03期
基金：
新加坡国家研究基金会;
关键词：
in vivo imaging; Lactococcus lactis; luciferase; pMG36e; recombinant DNA; GREEN FLUORESCENT PROTEIN; PROBIOTICS; BACTERIA; STRAIN;
D O I：
10.1007/s10068-012-0120-3
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
A recombinant DNA, pMG36e::luc(+) (5.3 kb), was constructed and transferred to Escherichia coli MC1061 and Lactococcus lactis MG1363 for in vivo imaging of the bacteria in murine intestines. E. coli MC1061 (pMG36e::luc(+)) and L. lactis MG1363 (pMG36e::luc(+)) displayed luciferase activities in vitro, where the bioluminescent signal of the E. coli was much stronger than that of the L. lactis by approximately 100-fold. These 2 recombinant bacteria were orally administered into rats. The bioluminescent signals of the E. coli and L. lactis in the gastrointestinal (GI) tracts of rats were detected and maintained up to 2 h via whole body imaging, indicating that the luciferase gene expression system for bacteria could be applied for in vivo imaging.
引用
收藏
页码：917 / 920
页数：4
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