In vivo imaging of Escherichia coli and Lactococcus lactis in murine intestines using a reporter luciferase gene

被引：6

作者：

Lee, Mi-Sook ^{[2
]}

Moon, Gi-Seong ^{[1
]}

机构：

[1] Chungju Natl Univ, Dept Biotechnol, Jeungpyeong 368701, Chungbuk, South Korea

[2] Korea Basic Sci Inst, Chuncheon Ctr, Div Bioimaging, Chunchon 200701, Gangwon, South Korea

来源：

FOOD SCIENCE AND BIOTECHNOLOGY | 2012年 / 21卷 / 03期

基金：

新加坡国家研究基金会;

关键词：

in vivo imaging; Lactococcus lactis; luciferase; pMG36e; recombinant DNA; GREEN FLUORESCENT PROTEIN; PROBIOTICS; BACTERIA; STRAIN;

D O I：

10.1007/s10068-012-0120-3

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A recombinant DNA, pMG36e::luc(+) (5.3 kb), was constructed and transferred to Escherichia coli MC1061 and Lactococcus lactis MG1363 for in vivo imaging of the bacteria in murine intestines. E. coli MC1061 (pMG36e::luc(+)) and L. lactis MG1363 (pMG36e::luc(+)) displayed luciferase activities in vitro, where the bioluminescent signal of the E. coli was much stronger than that of the L. lactis by approximately 100-fold. These 2 recombinant bacteria were orally administered into rats. The bioluminescent signals of the E. coli and L. lactis in the gastrointestinal (GI) tracts of rats were detected and maintained up to 2 h via whole body imaging, indicating that the luciferase gene expression system for bacteria could be applied for in vivo imaging.

引用

页码：917 / 920

页数：4

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