Use of SDS-PAGE of cell-wall proteins for rapid differentiation of Lactobacillus delbrueckii subsp lactis and Lactobacillus helveticus

被引：10
|
作者：
Hébert, EM
Raya, RR
de Giori, GS
机构：
[1] CONICET, CERELA, RA-4000 San Miguel De Tucuman, Tucuman, Argentina
[2] Univ Nacl Tucuman, Fac Bioquim Quim & Farm, Catedra Microbiol Super, RA-4000 San Miguel De Tucuman, Tucuman, Argentina
来源：
BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 12期
关键词：
cell-wall proteins; Lactobacillus; polyacrylamide gel electrophoresis;
D O I：
10.1023/A:1005645422298
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Cell-wall protein profiles of different strains of Lactobacillus helveticus and L. delbrueckii subsp. lactis isolated from regional cheeses were studied by SDS-PAGE. The patterns were highly reproducible and the presence of numerous bands with molecular weight ranging from 14 to 160 kDa allowed L. delbrueckii subsp. lactis to be differentiated from L. helveticus. The method is a reliable and rapid way to identify thermophilic lactobacilli.
引用
收藏
页码：1003 / 1006
页数：4
相关论文
共 48 条
12345