Use of SDS-PAGE of cell-wall proteins for rapid differentiation of Lactobacillus delbrueckii subsp lactis and Lactobacillus helveticus

被引：10

作者：

Hébert, EM

Raya, RR

de Giori, GS

机构：

[1] CONICET, CERELA, RA-4000 San Miguel De Tucuman, Tucuman, Argentina

[2] Univ Nacl Tucuman, Fac Bioquim Quim & Farm, Catedra Microbiol Super, RA-4000 San Miguel De Tucuman, Tucuman, Argentina

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 12期

关键词：

cell-wall proteins; Lactobacillus; polyacrylamide gel electrophoresis;

D O I：

10.1023/A:1005645422298

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cell-wall protein profiles of different strains of Lactobacillus helveticus and L. delbrueckii subsp. lactis isolated from regional cheeses were studied by SDS-PAGE. The patterns were highly reproducible and the presence of numerous bands with molecular weight ranging from 14 to 160 kDa allowed L. delbrueckii subsp. lactis to be differentiated from L. helveticus. The method is a reliable and rapid way to identify thermophilic lactobacilli.

引用

页码：1003 / 1006

页数：4

共 48 条

[31] Optimisation of Batch Culture Conditions for Cell-Envelope-Associated Proteinase Production from Lactobacillus delbrueckii subsp lactis ATCCA® 7830™
Agyei, Dominic
Potumarthi, Ravichandra
Danquah, Michael K.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2012, 168 (05) : 1035 - 1050
[32] Incorporation of Lactobacillus delbrueckii subsp lactis (CIDCA 133) in cold-set gels made from high pressure-treated soybean proteins
Hugo, Ayelen A.
Perez, Pablo F.
Cristina Anon, Maria
Speroni, Francisco
FOOD HYDROCOLLOIDS, 2014, 37 : 34 - 39
[33] SDS-POLYACRYLAMIDE GEL-ELECTROPHORESIS OF CELL-PROTEINS FROM LACTOBACILLUS-DELBRUECKII SUBSP BULGARICUS AND STREPTOCOCCUS-SALIVARIUS SUBSP THERMOPHILUS STRAINS ISOLATED FROM YOGURT AND CHEESE
TSAKALIDOU, E
ZOIDOU, E
KALANTZOPOULOS, G
MILCHWISSENSCHAFT-MILK SCIENCE INTERNATIONAL, 1992, 47 (05): : 296 - 298
[34] Optimisation of Batch Culture Conditions for Cell-Envelope-Associated Proteinase Production from Lactobacillus delbrueckii subsp. lactis ATCC® 7830™
Dominic Agyei
Ravichandra Potumarthi
Michael K. Danquah
Applied Biochemistry and Biotechnology, 2012, 168 : 1035 - 1050
[35] COMPARISON OF THE LACTOCOCCUS-LACTIS DIFFERENTIAL MEDIUM (DCL) AND SDS-PAGE OF WHOLE-CELL PROTEINS FOR THE IDENTIFICATION OF LACTOCOCCI TO SUBSPECIES LEVEL
DESCHEEMAEKER, P
POT, B
LEDEBOER, AM
VERRIPS, T
KERSTERS, K
SYSTEMATIC AND APPLIED MICROBIOLOGY, 1994, 17 (03) : 459 - 466
[36] CELL-WALL-ASSOCIATED PROTEINASE OF LACTOBACILLUS-DELBRUECKII SUBSP BULGARICUS CNRZ 397 - DIFFERENTIAL EXTRACTION, PURIFICATION AND PROPERTIES OF THE ENZYME
LALOI, P
ATLAN, D
BLANC, B
GILBERT, C
PORTALIER, R
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1991, 36 (02) : 196 - 204
[37] SDS-SOLUBILIZED WHOLE-CELL PROTEIN-PATTERNS OF STREPTOCOCCUS-SALIVARIUS SUBSP THERMOPHILUS AND LACTOBACILLUS-DELBRUECKII SUBSP BULGARICUS ISOLATED FROM GREEK YOGURTS
ZOURARI, A
COMMISSAIRE, J
DESMAZEAUD, MJ
JOURNAL OF DAIRY RESEARCH, 1992, 59 (01) : 105 - 109
[38] Potential of novel Lactobacillus helveticus strains and their cell wall bound proteases to release physiologically active peptides from milk proteins
Elfahri, K. R.
Donkor, O. N.
Vasiljevic, T.
INTERNATIONAL DAIRY JOURNAL, 2014, 38 (01) : 37 - 46
[39] Characterization of the pattern of αs1- and β-casein breakdown and release of a bioactive peptide by a cell envelope proteinase from Lactobacillus delbrueckii subsp lactis CRL 581"
Hebert, Elvira Maria
Mamone, Gianfranco
Picariello, Gianluca
Raya, Raul R.
Savoy, Graciela
Ferranti, Pasquale
Addeo, Francesco
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (12) : 3682 - 3689
[40] Rapid assessment of cell viability of Lactobacillus delbrueckii subsp. bulgaricus by measurement of intracellular pH in individual cells using fluorescence ratio imaging microscopy
Rechinger, KB
Siegumfeldt, H
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2002, 75 (1-2) : 53 - 60

← 1 2 3 4 5 →