A continuous cell line derived from larval fat bodies of Thysanoplusia intermixta (Lepidoptera: Noctuidae)

被引：11
|
作者：
Iwabuchi, K [1 ]
机构：
[1] Tokyo Univ Agr & Technol, Tokyo 1838509, Japan
来源：
APPLIED ENTOMOLOGY AND ZOOLOGY | 2000年 / 35卷 / 02期
关键词：
insect; cell culture; characterization; enzyme activity; karyotype;
D O I：
10.1303/aez.2000.245
中图分类号：
Q96 [昆虫学];
学科分类号：
摘要：
A continuous cell line has been established from larval fat bodies of a lepidopteran insect, Thysanoplusia intermixta. These cells were cultured in MGM-450 medium. The cell line, designated as TI-I, was a suspension of cells with a doubling time of 36 h and consisted mostly of spindle-shaped cells, although other shapes, notably spherical cells, also existed. The chromosome number of the cell line varied considerably with the mode of 150-179. This cell line was found to be susceptible to Autographa californica NPV (AcNPV). Isozyme analysis showed that the cells had distinctive patterns from other insect cell lines.
引用
收藏
页码：245 / 249
页数：5
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