New cell line established from pupal ovaries of Spodoptera litura F. (Lepidoptera: Noctuidae)

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作者：
Pant, U.
Athavale, S.S.
Basu, A.
Banerjee, K.
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Indian Journal of Experimental Biology | 1998年 / 36卷 / 02期
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Steady growth of cells from pupal ovaries of tobacco cutworm, S. litura was observed 107 days after initiation. Three cell types, viz. Epithelial-like cells (91%), fibroblast-like cells (7%) and giant cells (2%) could be distinguished. The cells grew loosely attached to the glass/plastic surface and could be easily dispersed by pipetting. The cells could be cryopreserved and at present are at 126th passage level. The chromosome number was high ranging from 41-200 and even more. Electron microscopy indicated the presence of virus-like particles in the nucleii of a fraction of the cell population. The cells were susceptible to nuclear polyhedrosis virus, Autographa californica.
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页码：195 / 198
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