A genetically encodable cell-type-specific protein synthesis inhibitor

被引：14
|
作者：
Heumueller, Maximilian [1 ]
Glock, Caspar [1 ]
Rangaraju, Vidhya [1 ]
Biever, Anne [1 ]
Schuman, Erin M. [1 ]
机构：
[1] Max Planck Inst Brain Res, Frankfurt, Germany
来源：
NATURE METHODS | 2019年 / 16卷 / 08期
基金：
欧洲研究理事会;
关键词：
RIBOSOME-INACTIVATING PROTEIN; VISUALIZATION;
D O I：
10.1038/s41592-019-0468-x
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Chemical inhibitors have revealed requirements for protein synthesis that drive cellular plasticity. We developed a genetically encodable protein synthesis inhibitor (gePSI) to achieve cell-type-specific temporal control of protein synthesis. Controlled expression of the gePSI in neurons or glia resulted in rapid, potent and reversible cell-autonomous inhibition of protein synthesis. Moreover, gePSI expression in a single neuron blocked the structural plasticity induced by single-synapse stimulation.
引用
收藏
页码：699 / +
页数：6
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