A genetically encodable cell-type-specific protein synthesis inhibitor

被引：14
|
作者：
Heumueller, Maximilian [1 ]
Glock, Caspar [1 ]
Rangaraju, Vidhya [1 ]
Biever, Anne [1 ]
Schuman, Erin M. [1 ]
机构：
[1] Max Planck Inst Brain Res, Frankfurt, Germany
来源：
NATURE METHODS | 2019年 / 16卷 / 08期
基金：
欧洲研究理事会;
关键词：
RIBOSOME-INACTIVATING PROTEIN; VISUALIZATION;
D O I：
10.1038/s41592-019-0468-x
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Chemical inhibitors have revealed requirements for protein synthesis that drive cellular plasticity. We developed a genetically encodable protein synthesis inhibitor (gePSI) to achieve cell-type-specific temporal control of protein synthesis. Controlled expression of the gePSI in neurons or glia resulted in rapid, potent and reversible cell-autonomous inhibition of protein synthesis. Moreover, gePSI expression in a single neuron blocked the structural plasticity induced by single-synapse stimulation.
引用
收藏
页码：699 / +
页数：6
相关论文
共 50 条
  • [1] A genetically encodable cell-type-specific protein synthesis inhibitor
    Maximilian Heumüller
    Caspar Glock
    Vidhya Rangaraju
    Anne Biever
    Erin M. Schuman
    Nature Methods, 2019, 16 : 699 - 702
  • [2] Author Correction: A genetically encodable cell-type-specific protein synthesis inhibitor
    Maximilian Heumüller
    Caspar Glock
    Vidhya Rangaraju
    Anne Biever
    Erin M. Schuman
    Nature Methods, 2019, 16 : 1332 - 1332
  • [3] A genetically encodable cell-type-specific protein synthesis inhibitor (vol 16, pg 699, 2019)
    Heumueller, Maximilian
    Glock, Caspar
    Rangaraju, Vidhya
    Biever, Anne
    Schuman, Erin M.
    NATURE METHODS, 2019, 16 (12) : 1332 - 1332
  • [4] Cell-type-specific quantification of protein synthesis in vivo
    Lorena Hidalgo San Jose
    Robert A. J. Signer
    Nature Protocols, 2019, 14 : 441 - 460
  • [5] Cell-type-specific quantification of protein synthesis in vivo
    San Jose, Lorena Hidalgo
    Signer, Robert A. J.
    NATURE PROTOCOLS, 2019, 14 (02) : 441 - 460
  • [6] Large-scale cell-type-specific imaging of protein synthesis in a vertebrate brain
    Shahar, Or David
    Schuman, Erin Margaret
    ELIFE, 2020, 9
  • [7] SPIDER: constructing cell-type-specific protein-protein interaction networks
    Kupershmidt, Yael
    Kasif, Simon
    Sharan, Roded
    BIOINFORMATICS ADVANCES, 2024, 4 (01):
  • [8] Multiple cell-type-specific elements regulate Myc protein stability
    Andreas Herbst
    Simone E Salghetti
    So Young Kim
    William P Tansey
    Oncogene, 2004, 23 : 3863 - 3871
  • [9] Multiple cell-type-specific elements regulate Myc protein stability
    Herbst, A
    Salghetti, SE
    Kim, SY
    Tansey, WP
    ONCOGENE, 2004, 23 (21) : 3863 - 3871
  • [10] Plakophilin 3 -: a novel cell-type-specific desmosomal plaque protein
    Schmidt, A
    Langbein, L
    Prätzel, S
    Rode, M
    Rackwitz, HR
    Franke, WW
    DIFFERENTIATION, 1999, 64 (05) : 291 - 306
12345