High-throughput single-strand conformation polymorphism analysis by capillary electrophoresis

被引：44
|
作者：
Ren, JC [1 ]
机构：
[1] Univ Bergen, Dept Pharmacol, N-5021 Bergen, Norway
来源：
JOURNAL OF CHROMATOGRAPHY B | 2000年 / 741卷 / 02期
关键词：
reviews; single-strand conformation polymorphism; mutation detection; polymerase chain reaction;
D O I：
10.1016/S0378-4347(00)00090-6
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Mutation detection plays a great role in genetic and medical research and clinical diagnosis of inherited diseases and particular cancers. Single-strand conformation polymorphism (SSCP) analysis is one of the most popular methods for detection of mutations. Recently, automated capillary electrophoresis (CE) systems have been used in SSCP analysis instead of conventional slab gel electrophoresis. SSCP analysis in combination with CE is a rapid, simple, sensitive and high-throughput mutation screening tool, and has been successfully applied for mutation detection involving human tumor suppressor genes, oncogenes and disease-causing genes. The new technique has a great potential for mutation screening of large numbers of samples in clinical diagnosis. This review discusses basic issues about the methodology of SSCP analysis based on CE and summarizes several key applications. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：115 / 128
页数：14
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