Proteomic analysis of prodigiosin-induced apoptosis in a breast cancer mitoxantrone-resistant (MCF-7 MR) cell line

被引:16
|
作者
Monge, Marta
Vilaseca, Marta
Soto-Cerrato, Vanessa
Montaner, Beatriz
Giralt, Ernest
Perez-Tomas, Ricardo
机构
[1] Univ Barcelona, Dept Patol & Terapeut Expt, CCBR Grp, E-08907 Barcelona, Spain
[2] Univ Barcelona, Serv Espectromet Masses Serv Cientificotecn, Barcelona, Spain
[3] Univ Barcelona, Dept Quim Organ, IRBB, PCB, Barcelona, Spain
关键词
apoptosis; prodigiosin; proteomics; breast cancer;
D O I
10.1007/s10637-006-7774-8
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Prodigiosin (PG) is a bacterial, red-pigmented antibiotic with immunosuppressive and apoptotic activities. To better understand its mechanisms of action, we tried to identify proteins associated with apoptosis induced by PG. For this purpose, the variation of protein expression on exposure to apoptotic concentrations of PG was examined, by high-resolution two-dimensional gel electrophoresis (2D-E), in the MCF-7 cancer cell line resistant to mitoxantrone (MCF-7-MR). Six PG apoptosis-associated protein spots were further characterized by complementary peptide mass fingerprinting and tandem mass spectrometry data obtained on a matrix-assisted laser desorption ionization-time-of-flight/time-of-flight (MALDI-TOF/TOF) mass spectrometer. The proteins identified were involved in various cellular functions, including cell defence, DNA repair and cellular organization. Our data provide novel information on cell response to PG, a new apoptotic drug with interesting anticancer activity.
引用
收藏
页码:21 / 29
页数:9
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