Alternative determination allergens in food by Real-Time PCR

被引:0
|
作者
Eugster, Albert [1 ]
机构
[1] Amt Verbraucherschutz Kantons Aargau, CH-5000 Aarau, Switzerland
关键词
POLYMERASE-CHAIN-REACTION;
D O I
暂无
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Nowadays many foods of plant origin with allergenic potential are analysed in foodstuffs by real-time PCR often in a multiplex manner [1]. These results are difficult to express in weight/weight units especially when composed food is analysed. This new method is able to determine contents of allergens in mass per cent based on a modified method of multiple standard addition with a simple mathematical analysis. It allows the quantitative analysis of an analyt in the range of 0.01 to 10 %
引用
收藏
页码:434 / 438
页数:5
相关论文
共 50 条
  • [41] The Making of "on-Chip PCR in Real-Time" for Food Quality Control
    Potrich C.
    Santini G.C.
    Lunelli L.
    Pasquardini L.
    Bertorelli R.
    de Sanctis V.
    Quattrone A.
    Pederzolli C.
    Potrich, C. (cpotrich@fbk.eu), 1600, Springer Science and Business Media, LLC (03): : 123 - 131
  • [42] SYBR Green Real-Time PCR Used to Detect Celery in Food
    Wu, Yajun
    Chen, Ying
    Wang, Bin
    Gao, Yunhua
    Bai, Liqun
    Wang, Haiyan
    JOURNAL OF AOAC INTERNATIONAL, 2010, 93 (05) : 1530 - 1536
  • [43] Food matrix standards for the quantification of allergenic food ingredients using real-time PCR
    Siegel, Marion
    Mutschler, A.
    Boernsen, B.
    Pietsch, Klaus
    Waiblinger, Hans-Ulrich
    EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2013, 237 (02) : 185 - 197
  • [44] Real-time PCR method for detection of pathogenic Yersinia enterocolitica in food
    Lambertz, S. Thisted
    Nilsson, C.
    Hallanvuo, S.
    Lindblad, M.
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (19) : 6060 - 6067
  • [45] Detection of pea in food by real-time polymerase chain reaction (PCR)
    B. Brežná
    L. Hudecová
    T. Kuchta
    European Food Research and Technology, 2006, 222 : 600 - 603
  • [46] Development and validation of a real-time PCR detection method for celery in food
    Hupfer, Christine
    Waiblinger, Hans-Ulrich
    Busch, Ulrich
    EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2007, 225 (3-4) : 329 - 335
  • [47] Development and validation of a real-time PCR detection method for celery in food
    Christine Hupfer
    Hans-Ulrich Waiblinger
    Ulrich Busch
    European Food Research and Technology, 2007, 225 : 329 - 335
  • [48] Application of Real-time Quantitative PCR Technology in Food Rapid Detection
    Ding B.
    Liu S.
    Science and Technology of Food Industry, 2021, 42 (07) : 366 - 373
  • [49] Food matrix standards for the quantification of allergenic food ingredients using real-time PCR
    Marion Siegel
    A. Mutschler
    B. Boernsen
    Klaus Pietsch
    Hans-Ulrich Waiblinger
    European Food Research and Technology, 2013, 237 : 185 - 197
  • [50] Real-time PCR for quantitative detection of bovine tissues in food and feed
    Martin, Irene
    Garcia, Teresa
    Fajardo, Violeta
    Rojas, Maria
    Hernandez, Pablo E.
    Gonzalez, Isabel
    Martin, Rosario
    JOURNAL OF FOOD PROTECTION, 2008, 71 (03) : 564 - 572