Semisynthetic tellurosubtilisin with glutathione peroxidase activity

被引：70
|
作者：
Mao, SZ [1 ]
Dong, ZY [1 ]
Liu, JQ [1 ]
Li, XQ [1 ]
Liu, XM [1 ]
Luo, GM [1 ]
Shen, JC [1 ]
机构：
[1] Jilin Univ, Key Lab Supramol Struct & Mat, Minist Educ, Changchun 130012, Peoples R China
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2005年 / 127卷 / 33期
关键词：
D O I：
10.1021/ja052451v
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Reaction of the hydroxyl group of serine-221 of subtilisin with phenylmethanesulfonylfluoride followed by nucleophilic substitution with sodium hydrogen telluride, a semisynthetic telluroprotein, tellurosubtilisin, was prepared. Tellurosubtilisin, which displays high substrate specificity for aromatic thiols, exhibits remarkable peroxidase activity and catalyzes the reduction of hydrogen peroxide by 3-carboxy-4-nitrobenzenethiol 20000 times more efficiently than diphenyl diselenide. Copyright © 2005 American Chemical Society.
引用
收藏
页码：11588 / 11589
页数：2
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