Development of reverse transcription loop mediated isothermal amplification assay for rapid detection of bluetongue viruses

被引：11
|
作者：
Mohandas, Sreekala S. [1 ]
Muthuchelvan, Dhanavelu [1 ]
Pandey, Awadh Bihari [1 ]
Biswas, Sanchay Kumar [1 ]
Chand, Karam [1 ]
Venkatesan, Gnanavel [1 ]
Choudhary, Dheeraj [1 ]
Ramakrishnan, Muthannan Andavar [1 ]
Mondal, Bimalendu [2 ]
机构：
[1] Indian Vet Res Inst, Div Virol, Mukteswar 263138, Uttarakhand, India
[2] Indian Vet Res Inst, Kolkata 700037, W Bengal, India
来源：
JOURNAL OF VIROLOGICAL METHODS | 2015年 / 222卷
关键词：
RT-LAMP; Isothermal amplification; Bluetongue; Ruminants; SEROTYPE; 21; BTV-21;
D O I：
10.1016/j.jviromet.2015.06.005
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A single-step reverse transcription loop mediated isothermal amplification (RT-LAMP) assay targeting NS1 - a highly conserved gene among BTV serotypes was optimized and validated with seven serotypes: BTV-1, BTV-2, BTV-9, BTV-10, BTV-16, BTV-21 and BTV-23. The relative sensitivity of the assay was 0.3 TCID50 and no cross reactivity could be observed with foot and mouth disease, peste-des-petits-ruminants, goatpox, sheeppox and orf viruses. The established assay was also assessed by screening of clinical samples and the result is comparable with conventional RT-PCR. The RT-LAMP assay described here could be an additional tool to the existing assays for diagnosis/surveillance of BTV. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：103 / 105
页数：3
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