Rapid detection of feline morbillivirus by a reverse transcription loop-mediated isothermal amplification

被引：3

作者：

Koide, Rie ^{[1
]}

Sakaguchi, Shoichi ^{[1
]}

Ogawa, Makoto ^{[2
]}

Miyazawa, Takayuki ^{[1
]}

机构：

[1] Kyoto Univ, Inst Virus Res, Dept Cell Biol, Lab Signal Transduct,Sakyo Ku, 53 Shogoin Kawaharacho, Kyoto 6068507, Japan

[2] Ogawa Pet Clin, 6-12 Shino Cho, Kameoka, Kyoto 6210822, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2016年 / 78卷 / 01期

基金：

日本学术振兴会;

关键词：

detection; feline; feline morbillivirus; reverse transcription loop-mediated isothermal amplification; CATS;

D O I：

10.1292/jvms.15-0239

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Feline morbillivirus (FmoPV) is an emerging virus in domestic cats and considered to be one of the causes of chronic renal failure in cats. In this study, we established a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of FmoPV. The results indicated that the detection limit of the assay was 10 50% tissue culture infective dose (TCID50)/ml in the original sample, and sensitivity of the assay was calculated as 0.12 TCID50 per one RT-LAMP reaction. We also detected FmoPV in clinical urine samples from cats infected with FmoPV. The FmoPV RT-LAMP assay is rapid, simple and highly specific for the detection of FmoPV, and thus, it would be a reliable detection method for FmoPV.

引用

页码：105 / 108

页数：4

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