Detection of pathogenesis-related proteins-chitinase and β-1,3-glucanase in induced chickpea

被引：0
|
作者：
Saikia, R [1 ]
Singh, BP [1 ]
Kumar, R [1 ]
Arora, DK [1 ]
机构：
[1] Natl Bur Agriculturally Important Microorganisms, Mau 275101, India
来源：
CURRENT SCIENCE | 2005年 / 89卷 / 04期
关键词：
chickpea; chitinase; beta-1,3-glucanase; pathogenesis-related proteins; phytopathogenic fungi;
D O I：
暂无
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Pathogenesis-related (PR) proteins, chitinase and beta-1,3-glucanase were extracted from induced chickpea plant and purified by gel filtration. Time-course accumulation of these PR-proteins in induced chickpea plants was significantly (P = 0.05) higher than the control. Maximum activities of these PR-proteins were recorded after three days of inoculation in all induced plants. Thereafter, the activity decreased progressively. Two chitinases and three beta-1,3-glucanases were detected in induced- chickpea plants. The molecular mass of the purified chitinases was 31 and 62 kDa and beta-1,3-glucanases was 23, 27 and 39 kDa. Purified chitinases and beta-1,3-glucanases also inhibited growth of Fusarium oxysporum f. sp. ciceri and other phytopathogenic fungi.
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页码：659 / 663
页数：5
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