Identification of a cDNA encoding 6-phosphogluconate dehydrogenase from a human heart cDNA library

被引：18
|
作者：
Tsui, SKW [1 ]
Chan, JYW [1 ]
Waye, MMY [1 ]
Fung, KP [1 ]
Lee, CY [1 ]
机构：
[1] CHINESE UNIV HONG KONG,DEPT BIOCHEM,SHATIN,NT,HONG KONG
来源：
BIOCHEMICAL GENETICS | 1996年 / 34卷 / 9-10期
关键词：
6-phosphogluconate dehydrogenase; carbon metabolism; pentose phosphate pathway; DNA sequence; Homo sapiens;
D O I：
10.1007/BF00554412
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A full-length cDNA clone for human 6-phosphogluconate dehydrogenase (PGD) was isolated from a human adult heart cDNA library. The clone encoded an open reading frame of 483 amino acids. When the amino acid sequences of human PGD and sheep PGD were aligned, 94.2% identity between these two proteins was found Its calculated molecular weight is 53,149 daltons. The predicted isoelectric point is 6.85. When the secondary structure of human PGD was examined by the PROSIS software, 36% alpha-helix and 9% beta-sheet were found.
引用
收藏
页码：367 / 373
页数：7
相关论文
共 50 条
  • [21] PURIFICATION AND PROPERTIES OF 6-PHOSPHOGLUCONATE DEHYDROGENASE FROM SHEEP LIVER
    VILLET, RH
    DALZIEL, K
    BIOCHEMICAL JOURNAL, 1967, 104 (03) : P38 - &
  • [22] KINETIC MECHANISM OF 6-PHOSPHOGLUCONATE DEHYDROGENASE FROM TORULA YEAST
    BERDIS, AJ
    COOK, PF
    FASEB JOURNAL, 1991, 5 (06): : A1512 - A1512
  • [23] A 6-PHOSPHOGLUCONATE DEHYDROGENASE GENE FROM TRYPANOSOMA-BRUCEI
    BARRETT, MP
    LEPAGE, RWF
    MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1993, 57 (01) : 89 - 100
  • [24] Selective inhibition of 6-phosphogluconate dehydrogenase from Trypanosoma brucei
    Bertelli, M
    El-Bastawissy, E
    Knaggs, MH
    Barrett, MP
    Hanau, S
    Gilbert, IH
    JOURNAL OF COMPUTER-AIDED MOLECULAR DESIGN, 2001, 15 (05) : 465 - 475
  • [25] Selective inhibition of 6-phosphogluconate dehydrogenase from Trypanosoma brucei
    Massimo Bertelli
    Eman El-Bastawissy
    Michael H. Knaggs
    Michael P. Barrett
    Stefania Hanau
    Ian H. Gilbert
    Journal of Computer-Aided Molecular Design, 2001, 15 : 465 - 475
  • [26] PURIFICATION AND PROPERTIES OF 6-PHOSPHOGLUCONATE DEHYDROGENASE FROM SHEEP LIVER
    VILLET, RH
    DALZIEL, K
    BIOCHEMICAL JOURNAL, 1969, 115 (04) : 639 - &
  • [27] ADDITIONAL ELECTROPHORETIC VARIANTS OF 6-PHOSPHOGLUCONATE DEHYDROGENASE
    BLAKE, NM
    SAHA, N
    MCDERMID, EM
    KIRK, RL
    CRANE, GG
    HUMANGENETIK, 1974, 21 (04): : 347 - 354
  • [28] STUDY OF 6-PHOSPHOGLUCONATE DEHYDROGENASE IN ROMAN POPULATION
    LUCARELL.P
    IANNETTI, P
    PALMARIN.R
    RUBERTO, U
    SCARABIN.R
    ATTI DELLA ACCADEMIA NAZIONALE DEI LINCEI RENDICONTI-CLASSE DI SCIENZE FISICHE-MATEMATICHE & NATURALI, 1973, 54 (01): : 139 - 142
  • [29] TRANSSPECIFIC VARIABILITY OF 6-PHOSPHOGLUCONATE DEHYDROGENASE IN PRIMATES
    TARIVERDIAN, G
    SCHMITT, J
    RITTER, H
    HUMANGENETIK, 1971, 11 (04): : 323 - +
  • [30] 6-phosphogluconate dehydrogenase deficiency in an Italian family
    Caprari, P
    Caforio, MP
    Cianciulli, P
    Maffi, D
    Pasquino, MT
    Tarzia, A
    Amadori, S
    Salvati, AM
    ANNALS OF HEMATOLOGY, 2001, 80 (01) : 41 - 44
12345