Layered double hydroxide nanoparticles as an appealing nanoparticle in gene/plasmid and drug delivery system in C2C12 myoblast cells

被引:46
|
作者
Yazdani, Parivar [1 ]
Mansouri, Elham [1 ]
Eyvazi, Shirin [2 ]
Yousefi, Vahid [1 ]
Kahroba, Homan [3 ]
Hejazi, Mohammad Saeid [1 ,3 ,4 ]
Mesbahi, Asghar [1 ]
Tarhriz, Vahideh [1 ]
Abolghasemi, Mir Mahdi [5 ]
机构
[1] Tabriz Univ Med Sci, Biomed Inst, Mol Med Res Ctr, Tabriz 5166414766, Iran
[2] Shahid Beheshti Univ Med Sci, Sch Adv Technol Med, Dept Biotechnol, Tehran, Iran
[3] Tabriz Univ Med Sci, Fac Adv Med Sci, Dept Mol Med, Tabriz, Iran
[4] Tabriz Univ Med Sci, Fac Pharm, Tabriz, Iran
[5] Univ Maragheh, Dept Chem, Fac Sci, Maragheh 5518183111, Iran
关键词
Layered double hydroxide; nanomaterial; drug/gene delivery; pCEP4/Cdk9; CONTROLLED-RELEASE; INTERCALATION; ACID; DELAMINATION; MODULATION; NANOHYBRID; CHLORIDE; FILMS;
D O I
10.1080/21691401.2018.1559182
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Gene and drug delivery systems need crucial update in the issue of nanocarriers. Layered double hydroxides (LDHs) are known as biocompatible inorganic lamellar nanomaterials with versatile properties. In the present study, Zn/Al-LDH nanoparticle was synthesized and characterized by FTIR, XRD, SEM, TEM and Zeta potential tests and then intercalated with valproate and methyldopa by co-precipitation and ion exchange methods. These nanocarriers were applied as high activity nanolayers-based delivery systems. On the other hand, Zn/Al-LDH + plasmid/gene (pCEP4/Cdk9) evaluated on C2C12 myoblast cells. Co-operation loading indicated high efficiency of sorting and release of drugs. Additionally, the Real-Time PCR and Western blotting results for plasmid-gene (pCEP4/Cdk9) delivery showed that Zn/Al-LDH nanoparticles can be used as an effective carrier in cellular uptake and release of genes for gene therapy. Easy and cost-effective production of Zn/Al-LDH nanoparticles proposed them as potential alternatives for the traditional routs of drug/gene delivery.
引用
收藏
页码:436 / 442
页数:7
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