Layered double hydroxide nanoparticles as an appealing nanoparticle in gene/plasmid and drug delivery system in C2C12 myoblast cells

被引:46
|
作者
Yazdani, Parivar [1 ]
Mansouri, Elham [1 ]
Eyvazi, Shirin [2 ]
Yousefi, Vahid [1 ]
Kahroba, Homan [3 ]
Hejazi, Mohammad Saeid [1 ,3 ,4 ]
Mesbahi, Asghar [1 ]
Tarhriz, Vahideh [1 ]
Abolghasemi, Mir Mahdi [5 ]
机构
[1] Tabriz Univ Med Sci, Biomed Inst, Mol Med Res Ctr, Tabriz 5166414766, Iran
[2] Shahid Beheshti Univ Med Sci, Sch Adv Technol Med, Dept Biotechnol, Tehran, Iran
[3] Tabriz Univ Med Sci, Fac Adv Med Sci, Dept Mol Med, Tabriz, Iran
[4] Tabriz Univ Med Sci, Fac Pharm, Tabriz, Iran
[5] Univ Maragheh, Dept Chem, Fac Sci, Maragheh 5518183111, Iran
关键词
Layered double hydroxide; nanomaterial; drug/gene delivery; pCEP4/Cdk9; CONTROLLED-RELEASE; INTERCALATION; ACID; DELAMINATION; MODULATION; NANOHYBRID; CHLORIDE; FILMS;
D O I
10.1080/21691401.2018.1559182
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Gene and drug delivery systems need crucial update in the issue of nanocarriers. Layered double hydroxides (LDHs) are known as biocompatible inorganic lamellar nanomaterials with versatile properties. In the present study, Zn/Al-LDH nanoparticle was synthesized and characterized by FTIR, XRD, SEM, TEM and Zeta potential tests and then intercalated with valproate and methyldopa by co-precipitation and ion exchange methods. These nanocarriers were applied as high activity nanolayers-based delivery systems. On the other hand, Zn/Al-LDH + plasmid/gene (pCEP4/Cdk9) evaluated on C2C12 myoblast cells. Co-operation loading indicated high efficiency of sorting and release of drugs. Additionally, the Real-Time PCR and Western blotting results for plasmid-gene (pCEP4/Cdk9) delivery showed that Zn/Al-LDH nanoparticles can be used as an effective carrier in cellular uptake and release of genes for gene therapy. Easy and cost-effective production of Zn/Al-LDH nanoparticles proposed them as potential alternatives for the traditional routs of drug/gene delivery.
引用
收藏
页码:436 / 442
页数:7
相关论文
共 50 条
  • [31] MyoD is regulated by the miR-29a-Tet1 pathway in C2C12 myoblast cells
    Chikenji, Akiyoshi
    Ando, Hitoshi
    Nariyama, Megumi
    Suga, Takeo
    Iida, Ryohei
    Gomi, Kazuhiro
    JOURNAL OF ORAL SCIENCE, 2016, 58 (02) : 219 - 229
  • [32] Vangl2 overexpression/silencing increases apoptosis and effects inflammation in C2C12 myoblast cells
    Lindqvist, M.
    Koho, S.
    Lindholm, D.
    Sejersen, T.
    HUMAN GENE THERAPY, 2016, 27 (11) : A57 - A57
  • [33] Melatonin-induced autophagy is associated with degradation of MyoD protein in C2C12 myoblast cells
    Kim, Chi Hyun
    Kim, Kyung Hwan
    Yoo, Yeong-Min
    JOURNAL OF PINEAL RESEARCH, 2012, 53 (03) : 289 - 297
  • [34] Ginkgolic acid regulates myogenic development by influencing the proliferation and differentiation of C2C12 myoblast cells
    Liu, Hyunju
    Joung, Hosouk
    MOLECULAR MEDICINE REPORTS, 2024, 30 (05)
  • [35] A new cell-based assay to evaluate myogenesis in mouse myoblast C2C12 cells
    Kodaka, Manami
    Yang, Zeyu
    Nakagawa, Kentaro
    Maruyama, Junichi
    Xu, Xiaoyin
    Sarkar, Aradhan
    Ichimura, Ayana
    Nasu, Yusuke
    Ozawa, Takeaki
    Iwasa, Hiroaki
    Ishigami-Yuasa, Mari
    Ito, Shigeru
    Kagechika, Hiroyuki
    Hata, Yutaka
    EXPERIMENTAL CELL RESEARCH, 2015, 336 (02) : 171 - 181
  • [36] On microRNA-214 suppressing osteogenic differentiation of C2C12 myoblast cells by targeting Osterix
    Gruenhagen, Johannes
    Ott, Claus-Eric
    BONE, 2013, 57 (01) : 325 - 327
  • [37] Sirtuin 1 promotes the proliferation of C2C12 myoblast cells via the myostatin signaling pathway
    Wang, Liang
    Zhang, Ting
    Xi, Yongyong
    Yang, Cuili
    Sun, Chengcao
    Li, Dejia
    MOLECULAR MEDICINE REPORTS, 2016, 14 (02) : 1309 - 1315
  • [38] Myostatin promotes tenogenic differentiation of C2C12 myoblast cells through Smad3
    Uemura, Kazutaka
    Hayashi, Masanori
    Itsubo, Toshiro
    Oishi, Ayumu
    Iwakawa, Hiroko
    Komatsu, Masatoshi
    Uchiyama, Shigeharu
    Kato, Hiroyuki
    FEBS OPEN BIO, 2017, 7 (04): : 522 - 532
  • [39] Oleate Ameliorates Palmitate-Induced Impairment of Differentiative Capacity in C2C12 Myoblast Cells
    Xue, Mengjuan
    Zhang, Fan
    Ji, Xueying
    Yu, Huiyuan
    Jiang, Xin
    Qiu, Yixuan
    Yu, Jiaming
    Chen, Jie
    Yang, Fan
    Bao, Zhijun
    STEM CELLS AND DEVELOPMENT, 2021, 30 (05) : 289 - 300
  • [40] Ca - signaling activation by complex nucleotide substance (PriMed) in mouse myoblast cells c2c12
    Orlova, Elena V.
    Orlova, Valentina S.
    Baeva, Julia I.
    Schemyatovsky, Alexandr N.
    FASEB JOURNAL, 2010, 24