Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry

被引：13
|
作者：
Suchanova, Bohumila [2 ]
Tuma, Roman [1 ,2 ]
机构：
[1] Univ Leeds, Astbury Ctr Struct Mol Biol, Leeds LS2 9JT, W Yorkshire, England
[2] Univ Helsinki, Inst Biotechnol, FIN-00014 Helsinki, Finland
来源：
MICROBIAL CELL FACTORIES | 2008年 / 7卷 / 1期
关键词：
D O I：
10.1186/1475-2859-7-12
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Recent advances in protein mass spectrometry (MS) have enabled determinations of hydrogen deuterium exchange (HDX) in large macromolecular complexes. HDX-MS became a valuable tool to follow protein folding, assembly and aggregation. The methodology has a wide range of applications in biotechnology ranging from quality control for over-expressed proteins and their complexes to screening of potential ligands and inhibitors. This review provides an introduction to protein folding and assembly followed by the principles of HDX and MS detection, and concludes with selected examples of applications that might be of interest to the biotechnology community.
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页数：20
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