Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry

被引：13
|
作者：
Suchanova, Bohumila [2 ]
Tuma, Roman [1 ,2 ]
机构：
[1] Univ Leeds, Astbury Ctr Struct Mol Biol, Leeds LS2 9JT, W Yorkshire, England
[2] Univ Helsinki, Inst Biotechnol, FIN-00014 Helsinki, Finland
来源：
MICROBIAL CELL FACTORIES | 2008年 / 7卷 / 1期
关键词：
D O I：
10.1186/1475-2859-7-12
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Recent advances in protein mass spectrometry (MS) have enabled determinations of hydrogen deuterium exchange (HDX) in large macromolecular complexes. HDX-MS became a valuable tool to follow protein folding, assembly and aggregation. The methodology has a wide range of applications in biotechnology ranging from quality control for over-expressed proteins and their complexes to screening of potential ligands and inhibitors. This review provides an introduction to protein folding and assembly followed by the principles of HDX and MS detection, and concludes with selected examples of applications that might be of interest to the biotechnology community.
引用
收藏
页数：20
相关论文
共 50 条
  • [21] Hydrogen-Deuterium Exchange Mass Spectrometry: A Novel Structural Biology Approach to Structure, Dynamics and Interactions of Proteins and Their Complexes
    Ozohanics, Oliver
    Ambrus, Attila
    [J]. LIFE-BASEL, 2020, 10 (11): : 1 - 18
  • [22] Modeling native state ensembles and macromolecular complexes using hydrogen-deuterium exchange ensemble reweighting
    Kihn, Kyle C.
    Smith, Ally K.
    Bradshaw, Richard
    Forrest, Lucy R.
    Deredge, Daniel J.
    [J]. BIOPHYSICAL JOURNAL, 2022, 121 (03) : 299A - 299A
  • [23] Characterization of peptide folding nuclei by hydrogen/deuterium exchange-mass spectrometry
    Li, X
    Hood, RJ
    Wedemeyer, WJ
    Watson, JT
    [J]. PROTEIN SCIENCE, 2005, 14 (07) : 1922 - 1928
  • [24] Oligosaccharide structures studied by hydrogen-deuterium exchange and MALDI-TOF mass spectrometry
    Price, Neil P. J.
    [J]. ANALYTICAL CHEMISTRY, 2006, 78 (15) : 5302 - 5308
  • [25] Microtubule-drug interactions studied by Hydrogen-Deuterium exchange and mass spectrometry.
    Xiao, Hui
    Verdier-Pinard, Pascal
    Fernandez-Fuentes, Narcis
    Fiser, Andras
    Angeletti, Ruth H.
    Orr, George A.
    Horwitz, Susan B.
    [J]. CANCER RESEARCH, 2006, 66 (08)
  • [26] Receptor-binding hydrogen-deuterium exchange mass spectrometry as an additional measurement of biosimilarity
    Treuheit, Nicholas A.
    Crawford, Nicholas F.
    Maki, Steven
    Payne, Jason
    Allen, Jeff
    [J]. JOURNAL OF PHARMACEUTICAL INVESTIGATION, 2020, 50 (04) : 413 - 423
  • [27] Hydrogen-Deuterium Exchange Mass Spectrometry of Phosphorylation Induced Changes in Smooth Muscle Myosin
    Sen, K. Ilker
    Haldeman, Brian D.
    Brizendine, Richard
    Mercer, Roger
    Cremo, Christine R.
    Fajer, Piotr G.
    [J]. BIOPHYSICAL JOURNAL, 2013, 104 (02) : 452A - 452A
  • [28] Computational method allowing Hydrogen-Deuterium Exchange Mass Spectrometry at single amide Resolution
    Gessner, Chris
    Steinchen, Wieland
    Bedard, Sabrina
    Skinner, John J.
    Woods, Virgil L.
    Walsh, Thomas J.
    Bange, Gert
    Pantazatos, Dionysios P.
    [J]. SCIENTIFIC REPORTS, 2017, 7
  • [29] Hydrogen-deuterium exchange mass spectrometry to study interactions and conformational changes of proteins in paints
    Galluzzi, Francesca
    Chaignepain, Stephane
    Arslanoglu, Julie
    Tokarski, Caroline
    [J]. BIOPHYSICAL CHEMISTRY, 2022, 289
  • [30] Structural Characterization of Aquaporin-0 by Native and Hydrogen-Deuterium Exchange Mass Spectrometry
    Schey, Kevin L.
    White, Wendy L.
    Furman, Aryel E.
    Harvey, Sophie R.
    Ju, Yue
    Panczyk, Erin M.
    Wysocki, Vicki H.
    [J]. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2019, 60 (09)
12345