Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry

被引：0
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作者：
Bohumila Suchanova
Roman Tuma
机构：
[1] Institute of Biotechnology,
[2] University of Helsinki,undefined
[3] Astbury Centre for Structural Molecular Biology,undefined
[4] University of Leeds,undefined
来源：
Microbial Cell Factories | / 7卷
关键词：
Energy Landscape; Supercritical Fluid Chromatography; Ultra Performance Liquid Chromatography; Folding Intermediate; Deuterium Incorporation;
D O I：
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学科分类号：
摘要：
Recent advances in protein mass spectrometry (MS) have enabled determinations of hydrogen deuterium exchange (HDX) in large macromolecular complexes. HDX-MS became a valuable tool to follow protein folding, assembly and aggregation. The methodology has a wide range of applications in biotechnology ranging from quality control for over-expressed proteins and their complexes to screening of potential ligands and inhibitors. This review provides an introduction to protein folding and assembly followed by the principles of HDX and MS detection, and concludes with selected examples of applications that might be of interest to the biotechnology community.
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