In vitro antitubercular activity of extract and constituents from the stem bark of Disthemonanthus benthamianus

A new C-glycosylflavone, apigenin 7-methyl ether 6-C-[beta-xylopyranosyl-(1?3)-beta-glucopyranoside] named distemonanthoside was isolated from the stem bark of Distemonanthus benthamianus Baill., Fabaceae, along with six known compounds, sitosterol 3-O-beta-d-glucopyranoside, 4-methoxygallic acid, syringic acid, quercetin, 6-O-acetylvitexin, quercetin 3-O-beta-d-glucopyranoside. The structures of those compounds and others were determined through spectral analyses. Compounds distemonanthoside, sitosterol 3-O-beta-d-glucopyranoside, 4-methoxygallic acid and quercetin were tested against a clinical isolate strain of Mycobacterium tuberculosis AC 45; they exhibited good to moderate antitubercular activities with MIC values ranged from 31.25 to 125 mu g/ml.(c) 2017 Sociedade Brasileira de Farmacognosia. Published by Elsevier Editora Ltda.