Comparison of ELISA for the detection of porcine serum antibodies to non-structural proteins of foot-and-mouth disease virus

被引：13
|
作者：
Lee, F [1 ]
Lin, YL [1 ]
Jong, MH [1 ]
机构：
[1] Anim Hlth Res Inst, Dept Hog Cholera Res, Tamsui 251, Taipei, Taiwan
来源：
JOURNAL OF VIROLOGICAL METHODS | 2004年 / 116卷 / 02期
关键词：
foot-and-mouth disease; non-structural protein; ELISA;
D O I：
10.1016/j.jviromet.2003.11.004
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Three foot-and-mouth disease virus non-structural protein antibody detection kits, CHEKIT FMD-3ABC, UBI FMD NS EIA and DVIVR NSP ELISA, were compared in the study. The results showed that the specificity of the kits ranged from 96.7 to 100% in naive pigs and from 93.6 to 98.1% in vaccinated pigs, and that the DVIVR kit had the highest analytical sensitivity. The kappa statistics for the detection of 612 sera were 0.582, 0.447 and 0.658 for CHEKIT/UBI, CHEKIT/DVIVR and UBI/DVIVR, respectively. This study also revealed that measurable non-structural protein specific antibodies in some of infected pigs were sustained either for shorter periods or in intermittent patterns, thus aggravating the difficulties associated with the removal of pre-exposed pigs in the field. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码：155 / 159
页数：5
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