Comparison of ELISA for the detection of porcine serum antibodies to non-structural proteins of foot-and-mouth disease virus

被引：13
|
作者：
Lee, F [1 ]
Lin, YL [1 ]
Jong, MH [1 ]
机构：
[1] Anim Hlth Res Inst, Dept Hog Cholera Res, Tamsui 251, Taipei, Taiwan
来源：
JOURNAL OF VIROLOGICAL METHODS | 2004年 / 116卷 / 02期
关键词：
foot-and-mouth disease; non-structural protein; ELISA;
D O I：
10.1016/j.jviromet.2003.11.004
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Three foot-and-mouth disease virus non-structural protein antibody detection kits, CHEKIT FMD-3ABC, UBI FMD NS EIA and DVIVR NSP ELISA, were compared in the study. The results showed that the specificity of the kits ranged from 96.7 to 100% in naive pigs and from 93.6 to 98.1% in vaccinated pigs, and that the DVIVR kit had the highest analytical sensitivity. The kappa statistics for the detection of 612 sera were 0.582, 0.447 and 0.658 for CHEKIT/UBI, CHEKIT/DVIVR and UBI/DVIVR, respectively. This study also revealed that measurable non-structural protein specific antibodies in some of infected pigs were sustained either for shorter periods or in intermittent patterns, thus aggravating the difficulties associated with the removal of pre-exposed pigs in the field. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码：155 / 159
页数：5
相关论文
共 50 条
  • [21] Differential distribution of non-structural proteins of foot-and-mouth disease virus in BHK-21 cells
    Garcia-Briones, Mercedes
    Rosas, Maria F.
    Gonzalez-Magaldi, Monica
    Martin-Acebes, Miguel A.
    Sobrino, Francisco
    Armas-Portela, Rosario
    VIROLOGY, 2006, 349 (02) : 409 - 421
  • [22] Differentiation of infection from vaccination by detection of antibodies to the non-structural protein 3ABC of foot-and-mouth disease virus
    Niedbalski, W
    Haas, B
    BULLETIN OF THE VETERINARY INSTITUTE IN PULAWY, 2003, 47 (01) : 51 - 60
  • [23] Comparison of sensitivity and specificity in three commercial foot-and-mouth disease virus non-structural protein ELISA kits with swine sera in Taiwan
    Chen, S. P.
    Ellis, T. M.
    Lee, M. C.
    Cheng, I. C.
    Yang, P. C.
    Lin, Y. L.
    Jong, M. H.
    Robertson, I. D.
    Edwards, J. R.
    VETERINARY MICROBIOLOGY, 2007, 119 (2-4) : 164 - 172
  • [24] Generation of Monoclonal Antibodies against Non-structural Protein 3AB of Foot-and-Mouth Disease Virus
    Tong Lin
    Virologica Sinica, 2012, (05) : 316 - 319
  • [25] Generation of Monoclonal Antibodies against Non-structural Protein 3AB of Foot-and-Mouth Disease Virus
    Tong Lin Junjun Shao Huiyun Chang Shandian Gao Guozheng Cong and Junzheng Du State Key Laboratory of Veterinary Etiological Biology National Foot and Mouth Disease Reference Laboratory Lanzhou Vetersinary Research Institute Chinese Academy of Agricultural Sciences Lanzhou China
    Virologica Sinica, 2012, 27 (05) : 316 - 319
  • [26] Production and application of recombinant antibodies to foot-and-mouth disease virus non-structural protein 3ABC
    Foord, Adam J.
    Muller, Janine D.
    Yu, Meng
    Wang, Lin-Fa
    Heine, Hans G.
    JOURNAL OF IMMUNOLOGICAL METHODS, 2007, 321 (1-2) : 142 - 151
  • [27] Detection of antibodies against structural proteins of foot-and-mouth disease virus in bovines of western Uttar India
    Afroz, Afroz
    Verma, Amit Kumar
    Kumar, Amit
    Upadhyay, Surendra
    Singh, Arbind
    VETERINARY RESEARCH FORUM, 2024, 15 (05) : 219 - 222
  • [28] MAb to non-structural protein (3B) of Foot-and-mouth Disease Virus
    Yang, M
    HYBRIDOMA, 2005, 24 (03): : 165 - 165
  • [29] Cellular and humoral immunity to foot-and-mouth disease virus and its non-structural proteins in infected swine.
    Cook, A
    Angove, H
    CedilloBarron, L
    FosterCuevas, M
    Parkhouse, RME
    BIOCHEMICAL SOCIETY TRANSACTIONS, 1997, 25 (02) : S273 - S273
  • [30] Comparison of different ELISA methods for the detection of antibodies against foot-and-mouth disease virus (FMDV) type O
    Niedbalski, W
    BULLETIN OF THE VETERINARY INSTITUTE IN PULAWY, 2004, 48 (01) : 5 - 9
12345