A new blocking ELISA for detection of foot-and-mouth disease non-structural protein (NSP) antibodies in a broad host range

被引：7

作者：

Hosamani, Madhusudan ^{[1
]}

Gopinath, Shreya ^{[1
]}

Sreenivasa, B. P. ^{[1
]}

Behera, Subhasmita ^{[1
]}

Basagoudanavar, Suresh H. ^{[1
]}

Boora, Ashok ^{[2
]}

Bora, Durlav P. ^{[3
]}

Deka, Pankaj ^{[3
]}

Bhanuprakash, Veerakyathappa ^{[1
]}

Singh, Raj Kumar ^{[1
,4
]}

Sanyal, Aniket ^{[1
]}

Weerdmeester, Klaas ^{[5
]}

Dekker, Aldo ^{[5
]}

机构：

[1] ICAR Indian Vet Res Inst, Bengaluru 560024, Karnataka, India

[2] ICAR CIRB, Sirsa Rd, Hisar 125001, Haryana, India

[3] Assam Agr Univ, Coll Vet Sci, Dept Vet Microbiol, Khanapara 781022, Guwahati, India

[4] ICAR Directorate FMD, Naini Tal 263138, Uttarakhand, India

[5] Wageningen Biovet Res, Virol & Mol Biol, POB 65, NL-8200 AB Lelystad, Netherlands

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2022年 / 106卷 / 19-20期

关键词：

Foot-and-mouth disease; Monoclonal antibody; Blocking ELISA; Non-structural protein antibodies; LINKED-IMMUNOSORBENT-ASSAY; VIRUS TRANSMISSION; VACCINATION; 3ABC; DIFFERENTIATION; QUANTIFICATION; VALIDATION; INFECTION; ANIMALS; CATTLE;

D O I：

10.1007/s00253-022-12151-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Large-scale monitoring of foot-and-mouth disease (FMD) in livestock is imperative in an FMD control program. Detection of antibodies against non-structural proteins (NSP) of FMD virus (FMDV) is one of the best tools to estimate the prevalence of past infection; availability of such a well-validated test is therefore essential. Using a FMDV 3B protein-specific monoclonal antibody, we have developed a new NSP antibody blocking ELISA (10H9 bELISA) and validated it on large panels of sera from different susceptible species. The diagnostic sensitivity of the ELISA was 95% with a specificity of 98%, similar to the values found using a commercial kit (PrioCHECK FMD NS test). The 10H9 bELISA can be used in a broad range of FMD susceptible species making it a very useful tool in monitoring the foot-and-mouth disease control programs by detection of virus circulation in the vaccinated populations.

引用

页码：6745 / 6757

页数：13

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