A new blocking ELISA for detection of foot-and-mouth disease non-structural protein (NSP) antibodies in a broad host range

被引:7
|
作者
Hosamani, Madhusudan [1 ]
Gopinath, Shreya [1 ]
Sreenivasa, B. P. [1 ]
Behera, Subhasmita [1 ]
Basagoudanavar, Suresh H. [1 ]
Boora, Ashok [2 ]
Bora, Durlav P. [3 ]
Deka, Pankaj [3 ]
Bhanuprakash, Veerakyathappa [1 ]
Singh, Raj Kumar [1 ,4 ]
Sanyal, Aniket [1 ]
Weerdmeester, Klaas [5 ]
Dekker, Aldo [5 ]
机构
[1] ICAR Indian Vet Res Inst, Bengaluru 560024, Karnataka, India
[2] ICAR CIRB, Sirsa Rd, Hisar 125001, Haryana, India
[3] Assam Agr Univ, Coll Vet Sci, Dept Vet Microbiol, Khanapara 781022, Guwahati, India
[4] ICAR Directorate FMD, Naini Tal 263138, Uttarakhand, India
[5] Wageningen Biovet Res, Virol & Mol Biol, POB 65, NL-8200 AB Lelystad, Netherlands
关键词
Foot-and-mouth disease; Monoclonal antibody; Blocking ELISA; Non-structural protein antibodies; LINKED-IMMUNOSORBENT-ASSAY; VIRUS TRANSMISSION; VACCINATION; 3ABC; DIFFERENTIATION; QUANTIFICATION; VALIDATION; INFECTION; ANIMALS; CATTLE;
D O I
10.1007/s00253-022-12151-2
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Large-scale monitoring of foot-and-mouth disease (FMD) in livestock is imperative in an FMD control program. Detection of antibodies against non-structural proteins (NSP) of FMD virus (FMDV) is one of the best tools to estimate the prevalence of past infection; availability of such a well-validated test is therefore essential. Using a FMDV 3B protein-specific monoclonal antibody, we have developed a new NSP antibody blocking ELISA (10H9 bELISA) and validated it on large panels of sera from different susceptible species. The diagnostic sensitivity of the ELISA was 95% with a specificity of 98%, similar to the values found using a commercial kit (PrioCHECK FMD NS test). The 10H9 bELISA can be used in a broad range of FMD susceptible species making it a very useful tool in monitoring the foot-and-mouth disease control programs by detection of virus circulation in the vaccinated populations.
引用
收藏
页码:6745 / 6757
页数:13
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