An amperometric biosensor for choline determination prepared from choline oxidase immobilized in polypyrrole-polyvinylsulfonate film

被引：9
|
作者：
Ozdemir, Merve [1 ]
Arslan, Fatma [1 ]
Arslan, Halit [1 ]
机构：
[1] Gazi Univ, Fac Arts & Sci, Dept Chem, Ankara, Turkey
来源：
ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY | 2012年 / 40卷 / 04期
关键词：
choline; choline oxidase; biosensor; polypyrrole (PPy); polyvinylsulphonate (PVS); CONDUCTING POLYMERS; PLATINUM-ELECTRODE; BEHAVIOR;
D O I：
10.3109/10731199.2011.646411
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
In this paper, a novel amperometric choline biosensor with immobilization of choline oxidase on electrochemically polymerized polypyrrole-polyvinylsulphonate (PPy-PVS) film has been accomplished via the entrapment technique. The effects of pH and temperature were investigated and optimum parameters were found to be 9.0 and 60 degrees C, respectively. There are two linear parts in the region between 1.0 x 10(-7) -1.0 x 10(-6) M (R-2 = 0.997) and 1.0 x 10(-5) -1.0 x 10(-3) M (R-2 = 0.986). The storage stability and operation stability of the enzyme electrode were also studied.
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页码：280 / 284
页数：5
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