An amperometric biosensor for uric acid determination prepared from uricase immobilized in polyaniline-polypyrrole film

被引：91
|
作者：
Arslan, Fatma [1 ]
机构：
[1] Gazi Univ, Fac Arts & Sci, Dept Chem, TR-06500 Ankara, Turkey
来源：
SENSORS | 2008年 / 8卷 / 09期
关键词：
uric acid; uricase; biosensor; polypyrrole; polyaniline;
D O I：
10.3390/s8095492
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A new amperometric uric acid biosensor was developed by immobilizing uricase by a glutaraldehyde crosslinking procedure on polyaniline-polypyrrole (pani-ppy) composite film on the surface of a platinum electrode. Determination of uric acid was performed by the oxidation of enzymatically generated H2O2 at 0.4 V vs. Ag/AgCl. The linear working range of the biosensor was 2.5x10(-6)-8.5x10(-5) M and the response time was about 70 s. The effects of pH, temperature were investigated and optimum parameters were found to be 9.0, 55 degrees C, respectively. The stability and reproducibility of the enzyme electrode have been also studied.
引用
收藏
页码：5492 / 5500
页数：9
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