4Pi confocal microscopy with alternate interference

被引：17

作者：

Hell, SW ^{[1
]}

Nagorni, M ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, High Resolut Opt Microscopy Grp, D-37070 Gottingen, Germany

来源：

OPTICS LETTERS | 1998年 / 23卷 / 20期

关键词：

D O I：

10.1364/OL.23.001567

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We introduce 4Pi confocal microscopy with destructive interference of converging waves. Linear lobe deconvolution as well as nonlinear restoration of 4Pi confocal raw data with their point-spread functions (PSF's) leads to almost-identical images, irrespective of whether the 4Pi confocal PSF relies on constructive or destructive interference. Three-dimensional imaging of microtubules of a mouse fibroblast cell yielded an axial resolution near 100-nm in both cases. Moreover, restoration of 4Pi confocal images of the same object with alternate phases is introduced as a powerful test for (nonlinear) image restoration. (C) 1998 Optical Society of America.

引用

页码：1567 / 1569

页数：3

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